Methyl Orange
Based on 1 Customer Validation
Methyl Orange is a soluble azo dye commonly used as an acid-base indicator and for staining cells and tissue sections, as well as for dyeing textiles. Methyl Orange appears red at a pH of 3.1 and changes to bright yellow as the pH increases to 4.4. Methyl Orange (500 mg/L) exhibits cytotoxicity and can cause DNA damage.
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- Purity: 99.85%
- CAS No.: 547-58-0
- 화학식: C14H14N3NaO3S
- 분자량:327.34
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보관:
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
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Biological Activity
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Chemical Information
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용액&용해도
- 순도&문서
- References
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Help & FAQs
Biological Activity
Guidelines (The following is our recommended experimental protocol. This protocol is for reference only, and specific operations should be adjusted according to your actual needs).
1. Acid-Base Indicator[2]
Methyl Orange exhibits a distinct color change at different pH values:
pH < 3.1: Red
pH 3.1 - 4.4: Orange
pH > 4.4: Bright Yellow
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 547-58-0
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Appearance Solid
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분자량 327.34
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화학식 C14H14N3NaO3S
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Color Orange to red
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SMILES
O=S(C1=CC=C(C=C1)/N=N/C2=CC=C(C=C2)N(C)C)(O[Na])=O
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선적
Room temperature in continental US; may vary elsewhere.
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보관
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
용액&용해도
DMSO : 20 mg/mL (61.10 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
순도&문서
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Data Sheet (271 KB)
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SDS (480 KB)
- English - EN (480 KB)
- Français - FR (480 KB)
- Deutsch - DE (480 KB)
- Norwegian - NO (480 KB)
- Español - ES (480 KB)
- Swedish - SV (480 KB)
- Italian - IT (480 KB)
- Korean - KR (480 KB)
- Portuguese - PT (480 KB)
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Handling Instructions (2659 KB)
References
[1]. Ahmad M, et al. Photocatalytic degradation of methyl orange from wastewater using a newly developed Fe-Cu-Zn-ZSM-5 catalyst. Environ Sci Pollut Res Int. 2020;27(21):26239-26248. [Content Brief]
[3]. Alaguprathana M, et al. Cytogenotoxicity assessment in Allium cepa roots exposed to methyl orange treated with Oedogonium subplagiostomum AP1. Environ Res. 2022 Oct;213:113612. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.0549 mL | 15.2746 mL | 30.5493 mL | 76.3732 mL |
| 5 mM | 0.6110 mL | 3.0549 mL | 6.1099 mL | 15.2746 mL | |
| 10 mM | 0.3055 mL | 1.5275 mL | 3.0549 mL | 7.6373 mL | |
| 15 mM | 0.2037 mL | 1.0183 mL | 2.0366 mL | 5.0915 mL | |
| 20 mM | 0.1527 mL | 0.7637 mL | 1.5275 mL | 3.8187 mL | |
| 25 mM | 0.1222 mL | 0.6110 mL | 1.2220 mL | 3.0549 mL | |
| 30 mM | 0.1018 mL | 0.5092 mL | 1.0183 mL | 2.5458 mL | |
| 40 mM | 0.0764 mL | 0.3819 mL | 0.7637 mL | 1.9093 mL | |
| 50 mM | 0.0611 mL | 0.3055 mL | 0.6110 mL | 1.5275 mL | |
| 60 mM | 0.0509 mL | 0.2546 mL | 0.5092 mL | 1.2729 mL |
Methyl Orange Related Classifications
HY-118907 Related Classifications
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