YMU1
Based on 1 Customer Validation
YMU1 is a selective inhibitor of human thymidylate kinase (hTMPK) with an IC50 of 610 nM. YMU1 inhibits tumor proliferation.
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- Purity : 99.19%
- CAS No.: 902589-96-2
- 화학식: C17H22N4O4S
- 분자량:378.45
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보관:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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용액&용해도
- Protocol
- 순도&문서
- References
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Help & FAQs
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HY-100614
AS1517499STAT6 Inhibitor -
HY-107430A
Oxythiamine chloride hydrochlorideTransketolase Inhibitor -
HY-19726
NSC59984MDM-2/p53 p53 Activator -
HY-100602
HJC0152STAT3 Inhibitor -
HY-108639
MIRA-1Anticancer Agent -
HY-107430AR
Oxythiamine chloride hydrochloride (Standard)Transketolase Inhibitor -
HY-108639R
MIRA-1 (Standard)Reference Standard -
HY-100614R
AS1517499 (Standard)STAT6 Inhibitor -
HY-100602R
HJC0152 (Standard)STAT3 Inhibitor
Biological Activity
제품 설명
IC50 & Target
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STAT3 |
In Vitro
YMU1 (0.1-10 μM, 10 min) has no inhibitory effect on TK1[1].
YMU1 is not toxic to the genome and, similar to TMPK silencing, impaires DSB repair[1].
YMU1 (1-10 μM, 14 days) has little effect on cell growth in H184B5F5M10, MCF10A, MCF-7, and HCT-116 cells[1].
YMU1 does not inhibit dUTPase in vitro[1].
YMU1 sensitizes malignant tumor cells to doxorubicin in vitro[1].
YMU1 (3-10 μM, 72 h) inhibits A549 cell proliferation, migration, and invasion[2].
YMU1 (3-10 μM, 72 h) controls the activation of STAT3 signaling pathway in LUAD cells[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
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Cell Line:H184B5F5M10, MCF10A, MCF-7, HCT-116
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Concentration:1, 10 μM
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Incubation Time:14 days; twice a week
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Result:Had little effect on cell growth.
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Cell Line:A549
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Concentration:3, 10 μM
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Incubation Time:72 h
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Result:Decreased cell viability.
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Cell Line:A549
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Concentration:3, 10 μM
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Incubation Time:72 h
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Result:Inhibited cell migration.
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Cell Line:A549
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Concentration:3, 10 μM
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Incubation Time:72 h
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Result:Inhibited cell invasion.
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Cell Line:A549; LUAD
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Concentration:3, 10 μM
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Incubation Time:72 h
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Result:Increased E-Cadherin approximately 3-fold, reduced N-Cadherin, fibronectin, and vimentin by more than 50%, and significantly reduced pSTAT3 levels, while total STAT3 levels had no significant change.
In Vivo
YMU1 (3-10 mg/kg, intraperitoneal injection, once every two days, for 30 days) inhibits the growth of A549 xenograft tumors in mice[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:HCT-116 p53-/- cell transplantation model in nude mice[1]
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Dosage:5 mg/kg; 3 times a week; 25 days
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Administration:Intraperitoneal injection (i.p.)
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Result:Showed that tumors grew significantly slower in mice treated with doxorubicin.
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Animal Model:A549 nude mouse transplantation model[2]
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Dosage:3,10 mg/kg; once every two days; 30 days
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Administration:Intraperitoneal injection (i.p.)
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Result:Reduced tumor volume and weight in a dose-dependent manner.
Chemical Information
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CAS No. 902589-96-2
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Appearance Solid
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분자량 378.45
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화학식 C17H22N4O4S
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Color Off-white to light yellow
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SMILES
O=C(N1CCN(C(CN2SC3=NC(C)=CC(C)=C3C2=O)=O)CC1)OCC
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선적
Room temperature in continental US; may vary elsewhere.
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보관
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
용액&용해도
In Vitro:
DMSO : 5.56 mg/mL (14.69 mM; ultrasonic and warming and heat to 70°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Protocol
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Cell Viability Determination by MTT Colorimetric Assay
The following protocol uses the MTT colorimetric assay as a classic literature-established method for assessing cell viability/metabolic activity in cultured mammalian cells. MTT[3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] is reduced by metabolically active cells to a colored formazan product; the amount of formazan is quantified spectrophotometrically and provides an indirect measure of metabolically active viable cells. Importantly, MTT reduction reflects cellular oxidoreductase/metabolic activity rather than an absolute direct count of living cells, so changes in cellular metabolism can alter the signal independently of cell number.
순도&문서
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Data Sheet (276 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 2.6424 mL | 13.2118 mL | 26.4236 mL | 66.0589 mL |
| 5 mM | 0.5285 mL | 2.6424 mL | 5.2847 mL | 13.2118 mL | |
| 10 mM | 0.2642 mL | 1.3212 mL | 2.6424 mL | 6.6059 mL |
YMU1 Related Classifications
HY-129125 Related Classifications
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