Cat. No.: HY-P10673

KwFwLL-NH2

KwFwLL-NH2, a hexapeptide, is a ghrelin receptor ligand. KwFwLL-NH2 can act as a specific inverse agonist at the ghrelin receptor but with moderate potency (EC₅₀=45.6 nM).

For research use only. We do not sell to patients.

CAS No.: 942266-38-8
Formula: C₄₉H₆₆N₁₀O₆
Molecular Weight:891.11

Storage:

Please store the product under the recommended conditions in the Certificate of Analysis.

Data Sheet (257 KB)

KwFwLL-NH2 Chemical Structure

CAS No.: 942266-38-8

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Biological Activity
- Description
- Cellular Effect
Chemical Information
Purity & Documentation
References
Calculators
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Biological Activity

Description

KwFwLL-NH2, a hexapeptide, is a ghrelin receptor ligand. KwFwLL-NH2 can act as a specific inverse agonist at the ghrelin receptor but with moderate potency (EC₅₀=45.6 nM)^[1].

All GHSR Isoforms :

GHSR1a

Cellular Effect

Cell Line	Type	Value	Description	References
COS-7	IC₅₀	>1000 nM Compound: KwFwLL-NH2	Agonist activity at human ghrelin receptor Asp2:20Lys(Asp99) mutant expressed in african green monkey COS7 cells assessed as inhibition of inositol phosphate accumulation Agonist activity at human ghrelin receptor Asp2:20Lys(Asp99) mutant expressed in african green monkey COS7 cells assessed as inhibition of inositol phosphate accumulation	[PMID: 17371869]
COS-7	IC₅₀	>1000 nM Compound: KwFwLL-NH2	Inverse agonist activity at human ghrelin receptor Arg6:20Gln(Arg283) mutant expressed in african green monkey COS7 cells assessed as inhibition of constitutively stimulated inositol phosphate accumulation Inverse agonist activity at human ghrelin receptor Arg6:20Gln(Arg283) mutant expressed in african green monkey COS7 cells assessed as inhibition of constitutively stimulated inositol phosphate accumulation	[PMID: 17371869]
COS-7	IC₅₀	>1000 nM Compound: KwFwLL-NH2	Inverse agonist activity at human ghrelin receptor Gln3:05Ala(Gln120) mutant expressed in african green monkey COS7 cells assessed as inhibition of constitutively stimulated inositol phosphate accumulation Inverse agonist activity at human ghrelin receptor Gln3:05Ala(Gln120) mutant expressed in african green monkey COS7 cells assessed as inhibition of constitutively stimulated inositol phosphate accumulation	[PMID: 17371869]
COS-7	IC₅₀	>1000 nM Compound: KwFwLL-NH2	Inverse agonist activity at human ghrelin receptor Glu3:09Gln(Glu124) mutant expressed in african green monkey COS7 cells assessed as inhibition of constitutively stimulated inositol phosphate accumulation Inverse agonist activity at human ghrelin receptor Glu3:09Gln(Glu124) mutant expressed in african green monkey COS7 cells assessed as inhibition of constitutively stimulated inositol phosphate accumulation	[PMID: 17371869]
COS-7	IC₅₀	>1000 nM Compound: KwFwLL-NH2	Inverse agonist activity at human ghrelin receptor Phe7:06Leu(Phe306) mutant expressed in african green monkey COS7 cells assessed as inhibition of constitutively stimulated inositol phosphate accumulation Inverse agonist activity at human ghrelin receptor Phe7:06Leu(Phe306) mutant expressed in african green monkey COS7 cells assessed as inhibition of constitutively stimulated inositol phosphate accumulation	[PMID: 17371869]
COS-7	IC₅₀	>1000 nM Compound: KwFwLL-NH2	Inverse agonist activity at human ghrelin receptor Phe7:09Ala(Phe312) mutant expressed in african green monkey COS7 cells assessed as inhibition of constitutively stimulated inositol phosphate accumulation Inverse agonist activity at human ghrelin receptor Phe7:09Ala(Phe312) mutant expressed in african green monkey COS7 cells assessed as inhibition of constitutively stimulated inositol phosphate accumulation	[PMID: 17371869]
COS-7	IC₅₀	13 nM Compound: KwFwLL-NH2	Displacement of [35S]MK677 from human ghrelin receptor expressed in african green monkey COS7 cells Displacement of [35S]MK677 from human ghrelin receptor expressed in african green monkey COS7 cells	[PMID: 17371869]
COS-7	IC₅₀	19 nM Compound: KwFwLL-NH2	Inverse agonist activity at human ghrelin receptor Phe5:12Ala(Phe220) mutant expressed in african green monkey COS7 cells assessed as inhibition of constitutively stimulated inositol phosphate accumulation Inverse agonist activity at human ghrelin receptor Phe5:12Ala(Phe220) mutant expressed in african green monkey COS7 cells assessed as inhibition of constitutively stimulated inositol phosphate accumulation	[PMID: 17371869]
COS-7	IC₅₀	2.6 nM Compound: KwFwLL-NH2	Inverse agonist activity at human ghrelin receptor Ser4:16Ala(Ser174) mutant expressed in african green monkey COS7 cells assessed as inhibition of constitutively stimulated inositol phosphate accumulation Inverse agonist activity at human ghrelin receptor Ser4:16Ala(Ser174) mutant expressed in african green monkey COS7 cells assessed as inhibition of constitutively stimulated inositol phosphate accumulation	[PMID: 17371869]
COS-7	IC₅₀	21 nM Compound: KwFwLL-NH2	Inverse agonist activity at human ghrelin receptor Ser3:08Ala(Ser123) mutant expressed in african green monkey COS7 cells assessed as inhibition of constitutively stimulated inositol phosphate accumulation Inverse agonist activity at human ghrelin receptor Ser3:08Ala(Ser123) mutant expressed in african green monkey COS7 cells assessed as inhibition of constitutively stimulated inositol phosphate accumulation	[PMID: 17371869]
COS-7	IC₅₀	27 nM Compound: KwFwLL-NH2	Inverse agonist activity at human ghrelin receptor Val5:08Ala(Val216) mutant expressed in african green monkey COS7 cells assessed as inhibition of constitutively stimulated inositol phosphate accumulation Inverse agonist activity at human ghrelin receptor Val5:08Ala(Val216) mutant expressed in african green monkey COS7 cells assessed as inhibition of constitutively stimulated inositol phosphate accumulation	[PMID: 17371869]
COS-7	IC₅₀	29 nM Compound: KwFwLL-NH2	Inverse agonist activity at human ghrelin receptor Met5:05Ala(Met213) mutant expressed in african green monkey COS7 cells assessed as inhibition of constitutively stimulated inositol phosphate accumulation Inverse agonist activity at human ghrelin receptor Met5:05Ala(Met213) mutant expressed in african green monkey COS7 cells assessed as inhibition of constitutively stimulated inositol phosphate accumulation	[PMID: 17371869]
COS-7	IC₅₀	32 nM Compound: KwFwLL-NH2	Inverse agonist activity at human wild type ghrelin receptor expressed in african green monkey COS7 cells assessed as inhibition of constitutively stimulated inositol phosphate accumulation Inverse agonist activity at human wild type ghrelin receptor expressed in african green monkey COS7 cells assessed as inhibition of constitutively stimulated inositol phosphate accumulation	[PMID: 17371869]
COS-7	IC₅₀	36 nM Compound: KwFwLL-NH2	Inverse agonist activity at human ghrelin receptor expressed in african green monkey COS7 cells assessed as constitutively stimulated inositol phosphate accumulation Inverse agonist activity at human ghrelin receptor expressed in african green monkey COS7 cells assessed as constitutively stimulated inositol phosphate accumulation	[PMID: 17371869]
COS-7	IC₅₀	43 nM Compound: KwFwLL-NH2	Inverse agonist activity at human ghrelin receptor Ile4:20Ala(Ile178) mutant expressed in african green monkey COS7 cells assessed as inhibition of constitutively stimulated inositol phosphate accumulation Inverse agonist activity at human ghrelin receptor Ile4:20Ala(Ile178) mutant expressed in african green monkey COS7 cells assessed as inhibition of constitutively stimulated inositol phosphate accumulation	[PMID: 17371869]
COS-7	IC₅₀	60 nM Compound: KwFwLL-NH2	Inverse agonist activity at human ghrelin receptor Phe3:04Ser(phe119) mutant expressed in african green monkey COS7 cells assessed as inhibition of constitutively stimulated inositol phosphate accumulation Inverse agonist activity at human ghrelin receptor Phe3:04Ser(phe119) mutant expressed in african green monkey COS7 cells assessed as inhibition of constitutively stimulated inositol phosphate accumulation	[PMID: 17371869]
COS-7	IC₅₀	61 nM Compound: KwFwLL-NH2	Inverse agonist activity at human ghrelin receptor Asn7:02Ala(Asn305) mutant expressed in african green monkey COS7 cells assessed as inhibition of constitutively stimulated inositol phosphate accumulation Inverse agonist activity at human ghrelin receptor Asn7:02Ala(Asn305) mutant expressed in african green monkey COS7 cells assessed as inhibition of constitutively stimulated inositol phosphate accumulation	[PMID: 17371869]
COS-7	IC₅₀	83 nM Compound: KwFwLL-NH2	Inverse agonist activity at human ghrelin receptor Ser5:09Ala(Ser217) mutant expressed in african green monkey COS7 cells assessed as inhibition of constitutively stimulated inositol phosphate accumulation Inverse agonist activity at human ghrelin receptor Ser5:09Ala(Ser217) mutant expressed in african green monkey COS7 cells assessed as inhibition of constitutively stimulated inositol phosphate accumulation	[PMID: 17371869]
COS-7	IC₅₀	>1000 nM Compound: KwFwLL-NH2	Agonist activity at human ghrelin receptor Asp2:20Asn(Asp99) mutant expressed in african green monkey COS7 cells assessed as inhibition of inositol phosphate accumulation Agonist activity at human ghrelin receptor Asp2:20Asn(Asp99) mutant expressed in african green monkey COS7 cells assessed as inhibition of inositol phosphate accumulation	[PMID: 17371869]
HEK-293T	EC₅₀	100 nM Compound: KwFwLL-NH2	Inverse agonist activity at human GHS-R1a expressed in HEK293T cells after 45 mins by HTRF-based IP3 turnover assay Inverse agonist activity at human GHS-R1a expressed in HEK293T cells after 45 mins by HTRF-based IP3 turnover assay	[PMID: 27072910]

Chemical Information

CAS No. 942266-38-8
Molecular Weight 891.11
Formula C₄₉H₆₆N₁₀O₆
SMILES

O=C(N[C@H](CC1=CNC2=CC=CC=C12)C(N[C@@H](CC3=CC=CC=C3)C(N[C@H](CC4=CNC5=CC=CC=C45)C(N[C@@H](CC(C)C)C(N[C@@H](CC(C)C)C(N)=O)=O)=O)=O)=O)[C@H](CCCCN)N
Sequence

Lys-{D-Trp}-Phe-{D-Trp}-Leu-Leu-NH2
Sequence Shortening

K-{D-Trp}-F-{D-Trp}-LL-NH2
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (257 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Els S, et al. An aromatic region to induce a switch between agonism and inverse agonism at the ghrelin receptor. J Med Chem. 2012 Sep 13;55(17):7437-49. [Content Brief]

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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KwFwLL-NH2

All GHSR Isoforms

Biological Activity

Chemical Information

Purity & Documentation

References

Calculators

Help & FAQs

KwFwLL-NH2 Related Classifications

HY-P10673 Related Classifications