LM11
LM11 is an inhibitor of transglutaminase 2 (TG2) with an activity of killing glioblastoma cells by maintaining TG2 in a cytotoxic conformational state.
For research use only. We do not sell to patients.
- Formula: C26H18Cl2N4O5
- Molecular Weight:537.35
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
In Vitro
LM11 (0.1-100 μM, 6 h) inhibits MDA-MB-231 cell migration in a dose-dependent fashion at stimulation with EGF.
LM11 (100 μM, 16 h) inhibits MDA-MB-231 cell viability with a 60% death rate.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
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Cell Line:MDA-MB-231
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Concentration:0.1, 1, 10, 100 μM
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Incubation Time:6 h
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Result:Inhibited cell migration at 10 uM of stimulation with EGF.
Inhibited cell viability at 100 uM after 16 h.
Chemical Information
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Molecular Weight 537.35
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Formula C26H18Cl2N4O5
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SMILES
O=C1NC(N(C(/C1=C/NC2=CC(CN3C(C4=C(C3=O)CCC=C4)=O)=C(C=C2)Cl)=O)C5=CC=CC(Cl)=C5)=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocols
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Cell Viability Determination by MTT Colorimetric Assay
The following protocol uses the MTT colorimetric assay as a classic literature-established method for assessing cell viability/metabolic activity in cultured mammalian cells. MTT[3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] is reduced by metabolically active cells to a colored formazan product; the amount of formazan is quantified spectrophotometrically and provides an indirect measure of metabolically active viable cells. Importantly, MTT reduction reflects cellular oxidoreductase/metabolic activity rather than an absolute direct count of living cells, so changes in cellular metabolism can alter the signal independently of cell number.
Purity & Documentation
References
[1]. Cody Aplin, et al. Defining the conformational states that enable transglutaminase 2 to promote cancer cell survival versus cell death. bioRxiv. 2024. [Content Brief]
[2]. Sabrina Schlienger, et al. ARF1 regulates adhesion of MDA-MB-231 invasive breast cancer cells through formation of focal adhesions. Cell Signal. 2015, 27, 3. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
LM11 Related Classifications
HY-157975 Related Classifications
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