Anti-Hepcidin/HAMP Antibody (LY2787106)

Cat. No.: HY-P990617 Purity: 98.23%: Data Sheet
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Anti-Hepcidin/HAMP Antibody (LY2787106) is a CHO-expressed human antibody that targets Hepcidin/HAMP.Anti-Hepcidin/HAMP Antibody (LY2787106) has a huIgG4SP type heavy chain and a huκ type light chain, with a predicted molecular weight (MW) of 144.2 kDa. The isotype control for Anti-Hepcidin/HAMP Antibody (LY2787106) can be referred to as Human IgG4 kappa, Isotype Control (HY-P99003).

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Description	Anti-Hepcidin/HAMP Antibody (LY2787106) is a CHO-expressed human antibody that targets Hepcidin/HAMP.Anti-Hepcidin/HAMP Antibody (LY2787106) has a huIgG4SP type heavy chain and a huκ type light chain, with a predicted molecular weight (MW) of 144.2 kDa. The isotype control for Anti-Hepcidin/HAMP Antibody (LY2787106) can be referred to as Human IgG4 kappa, Isotype Control (HY-P99003).
Isotype	Human IgG4 kappa
Recommend Isotype Controls	Human IgG4 (S228P) kappa, Isotype Control
Species Reactivity	Human
IC₅₀ & Target	Hepcidin/HAMP
Gene ID	57817 [NCBI]
Accession	P81172
Application	ELISA, FACS, Functional assay
Conjugated	Unconjugated
Reconsititution	The product can be reconstituted/diluted with sterile PBS or saline.
Molecular Weight	144.22 kDa
Appearance	Liquid
Color	Colorless to light yellow
SMILES	[Anti-Hepcidin/HAMP Antibody (LY2787106)]
Shipping	Shipping with dry ice.
Formulation	Please refer to the lot-specific COA for specific buffer information.
Storage	Please store the product under the recommended conditions in the Certificate of Analysis.
Biological Activity	Flow Cytometry analysis of HepG2 cells labelling Hepcidin/HAMP (red) with Hepcidin/HAMP Antibody (anti-Hepcidin/HAMP) (HY-P990617). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then cells were stained with the primary antibody at 1/200 dilution for an hour at 4℃. Goat Anti-Human IgG H&L (AF488) (HY-P83776) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃.Human IgG4 kappa isotype Control (HY-P99003, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black). Immobilized huHEPC/Hepcidin/HAMP-Fc can bind Anti-Hepcidin/HAMP Antibody (LY2787106). The EC₅₀ for this effect is 715.8 ng/mL.
Purity & Documentation	Purity: 98.23% Select Batch: Data Sheet (230 KB) SDS (251 KB) COA (232 KB) SDS-PAGE (58 KB) SEC-HPLC (102 KB) Inhibitory Antibodies User Guide (603 KB)

Anti-Hepcidin/HAMP Antibody (LY2787106) Related Classifications

Inflammation/Immunology

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Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Anti-Hepcidin/HAMP Antibody (LY2787106)OthersHepcidinSer/Thr ProteaseSerine proteasesSerine endopeptidasesThreonine proteasesHuman AntibodyHepcidin/HAMP AntibodyInhibitorinhibitorinhibit

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Flow Cytometry analysis of HepG2 cells labelling Hepcidin/HAMP (red) with Hepcidin/HAMP Antibody (anti-Hepcidin/HAMP) (HY-P990617). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then cells were stained with the primary antibody at 1/200 dilution for an hour at 4℃. Goat Anti-Human IgG H&L (AF488) (HY-P83776) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃.Human IgG4 kappa isotype Control (HY-P99003, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).
1/16

Immobilized huHEPC/Hepcidin/HAMP-Fc can bind Anti-Hepcidin/HAMP Antibody (LY2787106). The EC₅₀ for this effect is 715.8 ng/mL.
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