2. Immunology/Inflammation
  3. CD19
  4. LY3541860

LY3541860 is a selective anti-CD19 antibody that both binds human CD19 with high affinity and selectively binds human B cells (Kd values: 76.8 pM and 94.5 nM for humans and cynomolgus monkeys, respectively). LY3541860 inhibits CpG-induced CD69 expression (IC50 = 0.006 nM). LY3541860 exhibits potent B cell inhibitory activities. LY3541860 can inhibit both BCR- and TLR-induced human B cell activation. LY3541860 can be used for the research of autoimmune diseases.

For research use only. We do not sell to patients.

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LY3541860 Related Antibodies

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Description

LY3541860 is a selective anti-CD19 antibody that both binds human CD19 with high affinity and selectively binds human B cells (Kd values: 76.8 pM and 94.5 nM for humans and cynomolgus monkeys, respectively). LY3541860 inhibits CpG-induced CD69 expression (IC50 = 0.006 nM). LY3541860 exhibits potent B cell inhibitory activities. LY3541860 can inhibit both BCR- and TLR-induced human B cell activation. LY3541860 can be used for the research of autoimmune diseases[1].

Species Reactivity

Human
IC50 & Target

CD19
In Vitro

LY3541860 (1.6-1000 nM) binds human and cynomolgus CD19 with high affinity (Kd values: 76.8 pM, 94.5 nM, respectively[1].
LY3541860 (30 minutes) selectively binds human B cells in whole blood (EC50 = 0.184 ± 0.008 nM)[1].
LY3541860 (0.001 nM-10 nM; 30 min) inhibits B cell proliferation (IC50 = 0.008 nM)[1].
LY3541860 (0.1 pM-10 nM) can inhibit CpG-induced CD69 expression (IC50 = 0.006 nM)[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

LY3541860 Related Antibodies
In Vivo

LY3541860 (0.01-1.0 mg/kg; s.c.; weekly) inhibits B cell activation and immunoglobulin secretion in female humanized NSG mice (with a human IgM secretion attenuation of 57-78%)[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ (female, humanized with PBMC engraftment)[1]
Dosage: 0.01 mg/kg (IgM secretion attenuation); 1.0 mg/kg (IgM secretion attenuation and CD86 expression reduction)
Administration: s.c.; weekly
Result: Attenuated human IgM secretion by 60% on day 6 and 57% on day 10 at 0.01 mg/kg/week; attenuated IgM secretion by 78% on day 6 and 57% on day 10 at 1.0 mg/kg/week; reduced CD86 expression on human B cells in spleens dose-dependently
Clinical Trial
Gene ID

930  [NCBI]

Accession
Application

ELISA, FACS, Functional assay
Conjugated

Unconjugated
Reconsititution

The product can be reconstituted/diluted with sterile PBS or saline.
Appearance

Liquid

Color

Colorless to off-white

Shipping

Shipping with dry ice.
Formulation

Please refer to the lot-specific COA for specific buffer information.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.
Biological Activity
  • Immobilized Human CD19 (20-291) Protein, His Tag can bind LY3541860. The EC50 for this effect is 100 ng/mL.
Purity & Documentation
References
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LY3541860 Related Classifications

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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

LY3541860LY 3541860LY-3541860CD19AKT1humanized miceERK1/2B cell proliferationB cell differentiationWil2-s cellsBCR cappingprimary human B cellsB cell activationInhibitorinhibitorinhibit

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