LY3541860
Based on 1 Customer Validation
LY3541860 is a selective anti-CD19 antibody that both binds human CD19 with high affinity and selectively binds human B cells (Kd values: 76.8 pM and 94.5 nM for humans and cynomolgus monkeys, respectively). LY3541860 inhibits CpG-induced CD69 expression (IC50 = 0.006 nM). LY3541860 exhibits potent B cell inhibitory activities. LY3541860 can inhibit both BCR- and TLR-induced human B cell activation. LY3541860 can be used for the research of autoimmune diseases.
For research use only. We do not sell to patients.
- Purity: ≥99.0%
- Molecular Weight:144.704 kDa
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Human IgG4 kappa
Human
CD19
LY3541860 (1.6-1000 nM) binds human and cynomolgus CD19 with high affinity (Kd values: 76.8 pM, 94.5 nM, respectively[1].
LY3541860 (30 minutes) selectively binds human B cells in whole blood (EC50 = 0.184 ± 0.008 nM)[1].
LY3541860 (0.001 nM-10 nM; 30 min) inhibits B cell proliferation (IC50 = 0.008 nM)[1].
LY3541860 (0.1 pM-10 nM) can inhibit CpG-induced CD69 expression (IC50 = 0.006 nM)[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ (female, humanized with PBMC engraftment)[1]
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Dosage:0.01 mg/kg (IgM secretion attenuation); 1.0 mg/kg (IgM secretion attenuation and CD86 expression reduction)
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Administration:s.c.; weekly
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Result:Attenuated human IgM secretion by 60% on day 6 and 57% on day 10 at 0.01 mg/kg/week; attenuated IgM secretion by 78% on day 6 and 57% on day 10 at 1.0 mg/kg/week; reduced CD86 expression on human B cells in spleens dose-dependently
| NCT Number | Sponsor | Condition | Start Date |
Phase
|
|---|---|---|---|---|
| NCT01329991 | Plexxikon| | 2011-05 | PHASE1 |
Unconjugated
The product can be reconstituted/diluted with sterile PBS or saline.
ELISA, FACS, Functional assay
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Immobilized Human CD19 (20-291) Protein, His Tag can bind LY3541860. The EC50 for this effect is 100 ng/mL.
Chemical Information
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Appearance Liquid
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Molecular Weight 144.704 kDa
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Color Colorless to off-white
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Shipping
Shipping with dry ice.
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Formulation
Please refer to the lot-specific COA for specific buffer information.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
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Data Sheet (260 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Inhibitory Antibodies User Guide (603 KB)
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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