m7GpppAmpG ammonium solution (100 mM) (GMP like)
m7GpppAmpG ammonium solution (100 mM) (GMP-like) (m7G(5')ppp(5')(2'OMeA)pG solution (100 mM) (GMP-like)) is a GMP-like grade of m7GpppAmpG ammonium. m7GpppAmpG ammonium (m7G(5')ppp(5')(2'OMeA)pG ammonium) is a trinucleotide 5′ end cap analog. m7GpppAmpG ammonium binds to eIF4E with a KD value of 45.6 nM. m7GpppAmpG ammonium caps RNA with a capping efficiency of 90%. m7GpppAmpG ammonium enhances mRNA stability and translation efficiency. m7GpppAmpG ammonium is used in mRNA therapeutic research.
For research use only. We do not sell to patients.
- CAS No.: 3080642-79-8
- Formula: C32H43N15O24P4·xNH3
- Molecular Weight:1145.66 (free base)
-
Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Chemical Information
- Protocols
- Purity & Documentation
- References
-
Help & FAQs
All DNA/RNA Synthesis Isoforms
More
Biological Activity
Description
In Vitro
In Vivo
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
-
CAS No. 3080642-79-8
-
Molecular Weight 1145.66 (free base)
-
Formula C32H43N15O24P4·xNH3
-
SMILES
O=C1C2=C(N([C@H]3[C@H](O)[C@H](O)[C@@H](COP(OP(OP(OC[C@H]4O[C@@H](N(C=N5)C6=C5C(N)=NC=N6)[C@H](OC)[C@@H]4OP(OC[C@H]7O[C@@H](N(C=N8)C9=C8C(NC(N)=N9)=O)[C@H](O)[C@@H]7O)(O)=O)(O)=O)(O)=O)([O-])=O)O3)C=[N+]2C)N=C(N)N1.[x].N
-
Synonyms
m7G(5')ppp(5')(2'OMeA)pG solution (100 mM) (GMP like)
-
Shipping
Room temperature in continental US; may vary elsewhere.
-
Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocols
-
RNA extraction experimental
By lysing cells, releasing RNA, and removing impurities such as proteins and DNA, high-purity RNA products are finally obtained. The commonly used traditional method is the guanidine isothiocyanate/phenol/chloroform method (Trizol), which is suitable for a variety of animal materials including animal tissues, microorganisms, cultured cells, etc., and most plant materials.
Purity & Documentation
References
[1]. Sikorski PJ, Warminski M, Kubacka D, et al. The identity and methylation status of the first transcribed nucleotide in eukaryotic mRNA 5' cap modulates protein expression in living cells. Nucleic Acids Res. 2020;48(4):1607-1626. [Content Brief]
[3]. Raab M, et al. Rescue of p53 functions by in vitro-transcribed mRNA impedes the growth of high-grade serous ovarian cancer. Cancer Commun (Lond). 2024 Jan;44(1):101-126. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
m7GpppAmpG ammonium solution (100 mM) (GMP like) Related Classifications
HY-145974AGL Related Classifications
Powered by Bioz