Mal-Exatecan
Based on 1 Customer Validation
Mal-Exatecan is a maleimide (Mal)-modified Exatecan (HY-13631) and is a DNA topoisomerase I (topoisomerase I) inhibitor. Mal-Exatecan serves as a cytotoxic payload for antibody-drug conjugates (ADCs), conjugating to antibody cysteine residues via the maleimide group and releasing Exatecan within tumor cells to exert cytotoxic effects. Mal-Exatecan can be used for ADC construction and cancer-related research.
For research use only. We do not sell to patients.
- Purity : 99.39%
- CAS No.: 3028213-52-4
- Formula: C34H33FN4O7
- Molecular Weight:628.65
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Storage:
-20°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
IC50 & Target
topoisomerase I[1]
In Vitro
ADC (Antibody-Drug Conjugate) is composed of three parts: an antibody, a linker, and a cytotoxic drug. Through precise targeting by the antibody, it binds to antigens on the surface of target cells, enters the cells via endocytosis, and then releases the toxic drug, thereby achieving precise killing of target cells.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Chemical Information
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CAS No. 3028213-52-4
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Appearance Solid
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Molecular Weight 628.65
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Formula C34H33FN4O7
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Color Light yellow to yellow
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SMILES
O=C(C=CC1=O)N1CCCCCC(N[C@@H]2C3=C4C(C(N5C4)=CC([C@](O)(C(OC6)=O)CC)=C6C5=O)=NC7=CC(F)=C(C)C(CC2)=C73)=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
-20°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Solvent & Solubility
In Vitro:
DMSO : 100 mg/mL (159.07 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Protocols
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Cell Viability Determination by MTT Colorimetric Assay
The following protocol uses the MTT colorimetric assay as a classic literature-established method for assessing cell viability/metabolic activity in cultured mammalian cells. MTT[3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] is reduced by metabolically active cells to a colored formazan product; the amount of formazan is quantified spectrophotometrically and provides an indirect measure of metabolically active viable cells. Importantly, MTT reduction reflects cellular oxidoreductase/metabolic activity rather than an absolute direct count of living cells, so changes in cellular metabolism can alter the signal independently of cell number.
Purity & Documentation
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Data Sheet (293 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.5907 mL | 7.9536 mL | 15.9071 mL | 39.7678 mL |
| 5 mM | 0.3181 mL | 1.5907 mL | 3.1814 mL | 7.9536 mL | |
| 10 mM | 0.1591 mL | 0.7954 mL | 1.5907 mL | 3.9768 mL | |
| 15 mM | 0.1060 mL | 0.5302 mL | 1.0605 mL | 2.6512 mL | |
| 20 mM | 0.0795 mL | 0.3977 mL | 0.7954 mL | 1.9884 mL | |
| 25 mM | 0.0636 mL | 0.3181 mL | 0.6363 mL | 1.5907 mL | |
| 30 mM | 0.0530 mL | 0.2651 mL | 0.5302 mL | 1.3256 mL | |
| 40 mM | 0.0398 mL | 0.1988 mL | 0.3977 mL | 0.9942 mL | |
| 50 mM | 0.0318 mL | 0.1591 mL | 0.3181 mL | 0.7954 mL | |
| 60 mM | 0.0265 mL | 0.1326 mL | 0.2651 mL | 0.6628 mL | |
| 80 mM | 0.0199 mL | 0.0994 mL | 0.1988 mL | 0.4971 mL | |
| 100 mM | 0.0159 mL | 0.0795 mL | 0.1591 mL | 0.3977 mL |
Mal-Exatecan Related Classifications
HY-159783 Related Classifications
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