The Dimethylmethylene Blue Assay (DMMB) for the Quantification of Sulfated Glycosaminoglycans

  • Methods Mol Biol. 2023:2598:115-121. doi: 10.1007/978-1-0716-2839-3_9.
Yann D Ladner  1  2 ,  Mauro Alini  3 ,  Angela R Armiento  4
Affiliations
  • 1. AO Research Institute Davos, Davos Platz, Switzerland.
  • 2. Institute for Biomechanics, ETH Zurich, Zurich, Switzerland.
  • 3. AO Research Institute Davos, Davos Platz, Switzerland. [email protected].
  • 4. UCB Pharma, Slough, UK.
PMID: 36355288 DOI: 10.1007/978-1-0716-2839-3_9
Abstract

The 1,9-dimethylmethylene blue (DMMB) assay enables the detection of sulfated glycosaminoglycans (sGAGs). This assay can be used to quickly quantify the sGAG content in a large number of samples using spectrophotometry. While this widespread assay appears straightforward, there are certain pitfalls that need to be considered.

Keywords
Cartilage; DMMB; Dimethylmethylene blue assay; Sulfated glycosaminoglycans; sGAG.
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