Melatonin-OH

Cat. No.: HY-186080 Purity: 98.72%: Data Sheet
COA

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Handling Instructions
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Melatonin-OH is an oxidative Melatonin (HY-B0075) metabolite with free radical-scavenging activity. Melatonin-OH forms via Melatonin’s reaction with hydroxyl radicals (·OH). Melatonin-OH forms via Melatonin’s reaction with peroxynitrous acid or its activated form ONOOH.

For research use only. We do not sell to patients.

Melatonin-OH Chemical Structure

CAS No. : 67199-08-0

Size	Price	Stock
1 mg		Ask For Quote & Lead Time
5 mg		Ask For Quote & Lead Time

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

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Description

In Vitro

Melatonin-OH (Cyclic 3-hydroxymelatonin) (3COHM) (5 μg/mL) can be accurately detected and quantified via optimized HPLC, with a measured concentration of 4.93 μg/mL for a 5 μg/mL standard solution and a retention time of 8.1 min with excellent peak resolution^[1].
Cyclic 3-hydroxymelatonin (3COHM) (5 μg/mL) can be detected and quantified via optimized MEKC, with a measured concentration of 4.70 μg/mL for a 5 μg/mL standard solution, though results differ significantly from HPLC measurements^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

248.28

Formula

C₁₃H₁₆N₂O₃

CAS No.

67199-08-0

Appearance

Solid

Color

White to off-white

SMILES

O=C(C)N1CCC2(C3=CC(OC)=CC=C3NC12)O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility

In Vitro:

DMSO : ≥ 100 mg/mL (402.77 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	4.0277 mL	20.1386 mL	40.2771 mL
5 mM	0.8055 mL	4.0277 mL	8.0554 mL
10 mM	0.4028 mL	2.0139 mL	4.0277 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

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In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

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Working solution concentration: mg/mL

Purity & Documentation

Purity: 98.72%

Select Batch:

Data Sheet (275 KB) SDS (252 KB)

COA (252 KB) HNMR (1583 KB) RP-HPLC (1590 KB) MS (1013 KB)

Handling Instructions (2659 KB)

References

[1]. Hevia D, et al. Development and validation of new methods for the determination of melatonin and its oxidative metabolites by high performance liquid chromatography and capillary electrophoresis, using multivariate optimization. J Chromatogr A. 2010;1217(8):1368-1374. [Content Brief]

[2]. Galano A, et al. Melatonin as a natural ally against oxidative stress: a physicochemical examination. J Pineal Res. 2011;51(1):1-16. [Content Brief]

[3]. Reiter RJ, et al. Free radical-mediated molecular damage. Mechanisms for the protective actions of melatonin in the central nervous system. Ann N Y Acad Sci. 2001 Jun;939:200-15. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	4.0277 mL	20.1386 mL	40.2771 mL	100.6928 mL
	5 mM	0.8055 mL	4.0277 mL	8.0554 mL	20.1386 mL
	10 mM	0.4028 mL	2.0139 mL	4.0277 mL	10.0693 mL
	15 mM	0.2685 mL	1.3426 mL	2.6851 mL	6.7129 mL
	20 mM	0.2014 mL	1.0069 mL	2.0139 mL	5.0346 mL
	25 mM	0.1611 mL	0.8055 mL	1.6111 mL	4.0277 mL
	30 mM	0.1343 mL	0.6713 mL	1.3426 mL	3.3564 mL
	40 mM	0.1007 mL	0.5035 mL	1.0069 mL	2.5173 mL
	50 mM	0.0806 mL	0.4028 mL	0.8055 mL	2.0139 mL
	60 mM	0.0671 mL	0.3356 mL	0.6713 mL	1.6782 mL
	80 mM	0.0503 mL	0.2517 mL	0.5035 mL	1.2587 mL
	100 mM	0.0403 mL	0.2014 mL	0.4028 mL	1.0069 mL

Melatonin-OH Related Classifications

Inflammation/Immunology

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Melatonin-OH67199-08-0Drug MetaboliteONOOHmelatoninhydroxyl radicalsHPLCMEKCperoxynitrous acidInhibitorinhibitorinhibit

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Melatonin-OH

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