Methyl nicotinate (Synonyms: Nicotinic acid methyl ester)

Cat. No.: HY-B1695 Purity: 99.90%: FAQs
Data Sheet SDS COA Handling Instructions

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Methyl nicotinate (Nicotinic acid methyl ester) is an orally active vasodilator. Methyl nicotinate has analgesic activity. Methyl nicotinate causes local skin erythema when applied topically to the skin. Methyl nicotinate is used as an active ingredient in over-the-counter topical preparations for the study of muscle and joint pain.

For research use only. We do not sell to patients.

Methyl nicotinate Chemical Structure

CAS No. : 93-60-7

Size	Price	Stock	Quantity

Free Sample (0.1 - 0.5 mg)		Apply Now
Solid + Solvent
10 mM * 1 mL in DMSO ready for reconstitution	USD 55	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 55	In-stock	Estimated Time of Arrival: December 31
Solid
100 mg	USD 50	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
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Other Forms of Methyl nicotinate:

Methyl nicotinate-d₄ In-stock

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Biological Activity
Purity & Documentation
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Description

In Vitro

Methyl nicotinate (5 mg/mL; 24 h) is not cytotoxic in HDFn cells^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Cytotoxicity Assay^[2]

Cell Line:	HDFn (Human neonatal fibroblast cell line)
Concentration:	1 mg/mL, .2.5 mg/L, 5 mg/L, 7.5 mg/L and 10 mg/mL
Incubation Time:	24 h
Result:	Was extremely toxic to fibroblasts at 10mg/mL concentration, and 90% of the cells were still alive at5 mg/mL concentration.

In Vivo

Methyl nicotinate (5-10 mg.kg; Oral gavage; Single dose) has peripheral and central antialgesic activity in Swiss albino mice^[1].
Methyl nicotinate (5-10 mg/mL; 30-60 min) has vasodilatory effect in egg embryo eggs^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Writhing model of Swiss albino mice induced by acetic acid^[1]
Dosage:	5 mg/kg, 10 mg/kg
Administration:	Oral gavage; Single dose. Before Acetic acid treatment (10 mg/kg; i.p.; Single dose)
Result:	Caused a significant reduction in the number of writhes induced by acetic acid. At dose of 10 mg/kg, has a similar analgesic effect to Aspirin (HY-14654) within 30 minutes.

Animal Model:	Hot plate test Swiss albino mouse model^[1]
Dosage:	5 mg/kg, 10 mg/kg
Administration:	Oral gavage; Single dose
Result:	Could significantly prolong the latency of mice's response to thermal pain after 30 minutes of administration.

Animal Model:	Chorioallantoic Membrane (CAM) model^[2]
Dosage:	5 mg/mL/egg, 1 mg/mL/egg, 100 mg/mL/egg
Administration:
Result:	Promoted microvasodilation at 5 mg/mL/egg. Promoted vasodilation to a greater extent, but mild bleeding occurred at 10 mg/mL/egg. Caused extreme vasodilation, leading to blood vessel rupture and bleeding at 100 mg/mL/egg.

Molecular Weight

137.14

Formula

C₇H₇NO₂

CAS No.

93-60-7

Appearance

Solid

Color

White to off-white

SMILES

O=C(C1=CC=CN=C1)OC

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

H₂O : 100 mg/mL (729.18 mM; Need ultrasonic)

DMSO : 100 mg/mL (729.18 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	7.2918 mL	36.4591 mL	72.9182 mL
5 mM	1.4584 mL	7.2918 mL	14.5836 mL
10 mM	0.7292 mL	3.6459 mL	7.2918 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (18.23 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (18.23 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (18.23 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS
Solubility: 50 mg/mL (364.59 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99.93%

Select Batch:

Data Sheet (249 KB) SDS (0 KB)

COA (248 KB) HNMR (113 KB) RP-HPLC (134 KB)

Handling Instructions (2659 KB)

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O / DMSO	1 mM	7.2918 mL	36.4591 mL	72.9182 mL	182.2955 mL
	5 mM	1.4584 mL	7.2918 mL	14.5836 mL	36.4591 mL
	10 mM	0.7292 mL	3.6459 mL	7.2918 mL	18.2295 mL
	15 mM	0.4861 mL	2.4306 mL	4.8612 mL	12.1530 mL
	20 mM	0.3646 mL	1.8230 mL	3.6459 mL	9.1148 mL
	25 mM	0.2917 mL	1.4584 mL	2.9167 mL	7.2918 mL
	30 mM	0.2431 mL	1.2153 mL	2.4306 mL	6.0765 mL
	40 mM	0.1823 mL	0.9115 mL	1.8230 mL	4.5574 mL
	50 mM	0.1458 mL	0.7292 mL	1.4584 mL	3.6459 mL
	60 mM	0.1215 mL	0.6077 mL	1.2153 mL	3.0383 mL
	80 mM	0.0911 mL	0.4557 mL	0.9115 mL	2.2787 mL
	100 mM	0.0729 mL	0.3646 mL	0.7292 mL	1.8230 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Methyl nicotinate93-60-7Nicotinic acid methyl esterInhibitorinhibitorinhibit

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