1. Cell Cycle/DNA Damage
    Apoptosis
  2. DNA Alkylator/Crosslinker
    Apoptosis
  3. (-)-Irofulven

(-)-Irofulven (Synonyms: MGI 114; 6-Hydroxymethylacylfulvene; NSC 683863)

Cat. No.: HY-14429
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(-)-Irofulven (MGI 114), an Illudin S analog, is a DNA alkylating agent. (-)-Irofulven inhibits the replication of DNA, induces tumor cells apoptosis, and has potent antitumor activity.

For research use only. We do not sell to patients.

(-)-Irofulven Chemical Structure

(-)-Irofulven Chemical Structure

CAS No. : 158440-71-2

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Description

(-)-Irofulven (MGI 114), an Illudin S analog, is a DNA alkylating agent. (-)-Irofulven inhibits the replication of DNA, induces tumor cells apoptosis, and has potent antitumor activity[1][2].

In Vitro

(-)-Irofulven (2.8 μM; 1 hour) induces p53-dependent cell cycle arrest[1].
(-)-Irofulven (0.8 μM, 0.9 μM and 2.8 μM; 1 hour) induces CHK2 activation is related to p53 status in cells[1].
(-)-Irofulven inhibits DNA replication and induces chromosome aberrations (breaks and radials)[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Cycle Analysis[1]

Cell Line: A2780, CAOV3 and HCT116 cells
Concentration: 2.8 μM
Incubation Time: 1 hour
Result: p53 wild-type cells mainly arrested at G1/S phases, while p53-mutated or p53-null cells arrested at S and G2/M phases.

Western Blot Analysis[1]

Cell Line: A2780, CAOV3 and HCT116 cells
Concentration: 0.8 μM, 0.9 μM and 2.8 μM
Incubation Time: 1 hour
Result: Induced the Thr 68 phosphorylation of CHK2 kinase in cells.
In Vivo

(-)-Irofulven (7 mg/kg; i.p; on days 1-5 and 8-12) produces a statistically significant increase in the median survival of mice bearing tumor cells[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Male and female athymic BALB/c mice (nu/nu genotype, 6 weeks old or older) injected with human glioblastoma multiforme[2].
Dosage: 7 mg/kg
Administration: i.p; on days 1-5 and 8-12
Result: Was active against all tumor lines.
Molecular Weight

246.30

Formula

C₁₅H₁₈O₃

CAS No.
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(-)-Irofulven
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HY-14429
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