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  3. Mito Red

Mito Red is a vital dye and mitochondrial stain that can be used to detect and evaluate mitochondrial function and status. Mito Red accumulates in mitochondria, and its fluorescence intensity is positively correlated with mitochondrial membrane potential. When the mitochondrial membrane potential increases, the fluorescence signal of Mito Red increases.

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Mito Red

Mito Red Chemical Structure

CAS No. : 1021902-10-2

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Based on 1 publication(s) in Google Scholar

Top Publications Citing Use of Products

1 Publications Citing Use of MCE Mito Red

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Description

Mito Red is a vital dye and mitochondrial stain that can be used to detect and evaluate mitochondrial function and status. Mito Red accumulates in mitochondria, and its fluorescence intensity is positively correlated with mitochondrial membrane potential. When the mitochondrial membrane potential increases, the fluorescence signal of Mito Red increases[1].

Molecular Weight

637.16

Formula

C38H37ClN2O5

CAS No.
Appearance

Solid

Color

dark brown

SMILES

O=C1OC2=C(C(C)=C1)C=CC(OC(C3=C(C4=C5C=CC(N(CC)CC)=CC5=[O+]C6=C4C=CC(N(CC)CC)=C6)C=CC=C3)=O)=C2.[Cl-]

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 4.17 mg/mL (6.54 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.5695 mL 7.8473 mL 15.6946 mL
5 mM 0.3139 mL 1.5695 mL 3.1389 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo Dissolution Calculator
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Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.5695 mL 7.8473 mL 15.6946 mL 39.2366 mL
5 mM 0.3139 mL 1.5695 mL 3.1389 mL 7.8473 mL
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  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Product Name:
Mito Red
Cat. No.:
HY-D1359
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