MitoB bromide
Based on 1 Customer Validation
MitoB bromide is an exomarker of mitochondrial hydrogen peroxide. MitoB bromide is a mitochondria-targeted ratiometric probe, and can be used to measure levels of one major ROS, hydrogen peroxide, within living animals.
For research use only. We do not sell to patients.
- Purity: 99.8%
- CAS No.: 1247025-84-8
- Formula: C25H23BBrO2P
- Molecular Weight:477.14
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Storage:
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Biological Activity
MitoB is rapidly taken up by mitochondria and converts to MitoP, which enables the quantification of H2O2[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 1247025-84-8
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Appearance Solid
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Molecular Weight 477.14
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Formula C25H23BBrO2P
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Color White to off-white
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SMILES
OB(C1=CC=CC(C[P+](C2=CC=CC=C2)(C3=CC=CC=C3)C4=CC=CC=C4)=C1)O.[Br-]
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Solvent & Solubility
DMSO : 116.67 mg/mL (244.52 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (275 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
[1]. Salin K, et al. Using the MitoB method to assess levels of reactive oxygen species in ecological studies of oxidative stress. Sci Rep. 2017 Jan 24;7:41228. [Content Brief]
[2]. Cochemé HM, et al. Using the mitochondria-targeted ratiometric mass spectrometry probe MitoB to measure H2O2 in living Drosophila. Nat Protoc. 2012 Apr 19;7(5):946-58. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.0958 mL | 10.4791 mL | 20.9582 mL | 52.3955 mL |
| 5 mM | 0.4192 mL | 2.0958 mL | 4.1916 mL | 10.4791 mL | |
| 10 mM | 0.2096 mL | 1.0479 mL | 2.0958 mL | 5.2396 mL | |
| 15 mM | 0.1397 mL | 0.6986 mL | 1.3972 mL | 3.4930 mL | |
| 20 mM | 0.1048 mL | 0.5240 mL | 1.0479 mL | 2.6198 mL | |
| 25 mM | 0.0838 mL | 0.4192 mL | 0.8383 mL | 2.0958 mL | |
| 30 mM | 0.0699 mL | 0.3493 mL | 0.6986 mL | 1.7465 mL | |
| 40 mM | 0.0524 mL | 0.2620 mL | 0.5240 mL | 1.3099 mL | |
| 50 mM | 0.0419 mL | 0.2096 mL | 0.4192 mL | 1.0479 mL | |
| 60 mM | 0.0349 mL | 0.1747 mL | 0.3493 mL | 0.8733 mL | |
| 80 mM | 0.0262 mL | 0.1310 mL | 0.2620 mL | 0.6549 mL | |
| 100 mM | 0.0210 mL | 0.1048 mL | 0.2096 mL | 0.5240 mL |