MTS peptide
MTS peptide is a short peptide with a mitochondria-targeting sequence. In F-M-LNP, MTS peptide is displayed on the particle surface via thiol-maleimide conjugation, and is specifically responsible for being recognized by the mitochondrial proteins TOMM20/22, "anchoring" the nanoparticles to the outer mitochondrial membrane, followed by membrane potential-independent bilayer membrane penetration mediated by fluorinated lipids. MTS peptide can be used in the research of Leber's hereditary optic neuropathy.
Nur für Forschungszwecke. Wir verkaufen nicht an Patienten.
- Formel: C73H121N21O17S2
- Molecular Weight:1629.00
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Speicherung:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biologische Aktivität
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Chemical Information
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Biologische Aktivität
Beschreibung
In Vitro
MTS peptide modification enhances the mitochondrial targeting selectivity of 6F-LNPs in N2a cells by increasing interactions with TOMM20 and TOMM22, resulting in a Pearson's colocalization coefficient of 0.90 between delivered pDNA and mitochondria[1].
MTS peptide-modified 6F-M-LNPs (2 μg/mL; 24 h followed by 48 h fresh medium culture) deliver functional hND4 to GM10742 LHON patient cells, restoring mitochondrial function with a 5-fold increase in hND4 protein expression and significant improvements in MMP, ATP production, and oxygen consumption[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Chemical Information
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Molecular Weight 1629.00
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Formel C73H121N21O17S2
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SMILES
O=C(N[C@@H](CC(C)C)C(N[C@@H](CO)C(N[C@@H](CC(C)C)C(N[C@@H](CCCNC(N)=N)C(N[C@@H](CCC(N)=O)C(N[C@@H](CO)C(N[C@@H]([C@@H](C)CC)C(N[C@@H](CCCNC(N)=N)C(N[C@@H](CC1=CC=CC=C1)C(N[C@@H](CC2=CC=CC=C2)C(N[C@@H](CCCCN)C(N[C@@H](CS)C(O)=O)=O)=O)=O)=O)=O)=O)=O)=O)=O)=O)=O)[C@H](CCSC)N
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Sequence
Met-Leu-Ser-Leu-Arg-Gln-Ser-Ile-Arg-Phe-Phe-Lys-Cys
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Sequence Shortening
MLSLRQSIRFFKC
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Versand
Room temperature in continental US; may vary elsewhere.
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Speicherung
Please store the product under the recommended conditions in the Certificate of Analysis.
Protokoll
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Fluorescent plasma-membrane potential dye assay
Fluorescent plasma-membrane potential dye assays measure changes in cell membrane potential using voltage-sensitive dyes whose fluorescence changes when cells depolarize or hyperpolarize. Anionic bis-oxonol dyes such as DiBAC4(3) enter depolarized cells more readily and show increased fluorescence after intracellular binding, while hyperpolarization reduces dye accumulation and fluorescence. FMP/FLIPR membrane-potential dyes are used for faster, homogeneous microplate assays of ion-channel or receptor-mediated membrane-potential changes.
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Calculators
Konzentration (Stammlösung) × Volumen (Stammlösung) = Konzentration (Ziellösung) × Volumen (Ziellösung)
MTS peptide Related Classifications
HY-P12011 Related Classifications
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