Perosamine
Perosamine is a dideoxysugar that can be isolated from the O-antigens of Gram-negative bacteria, including Vibrio cholerae O1 and Escherichia coli O157:H7.
For research use only. We do not sell to patients.
- CAS No.: 31348-80-8
- Formula: C6H13NO4
- Molecular Weight:163.17
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
Biological Activity
Description
Chemical Information
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CAS No. 31348-80-8
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Molecular Weight 163.17
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Formula C6H13NO4
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SMILES
C[C@@H](O)[C@@H](N)[C@H](O)[C@H](O)C=O
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Synonyms
D-Perosamine
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Initial Source
Escherichia coli
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocols
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Gram Staining of Tissue Sections
Gram staining of tissue sections is a histochemical technique used to differentiate Gram-positive and Gram-negative bacteria within histological specimens based on differences in bacterial cell wall structure and dye retention, adapted from classical bacteriological Gram staining into tissue-compatible “histological Gram stain” variants. In tissue applications, modifications of the Brown-Hopps and Brown-Brenn methods are commonly used to improve differentiation of microorganisms embedded within host connective tissue and to reduce overstaining or loss of Gram-negative signal, which are known limitations of earlier approaches. The principle relies on crystal violet-iodine complex retention in Gram-positive organisms and subsequent decolorization and counterstaining steps that allow contrast visualization of Gram-negative organisms against tissue background.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)