N-Oxalylglycine
Based on 1 publication(s) in Google Scholar
N-Oxalylglycine (N-Oxaloglycine) is a Jumonji C-domain-containing histone lysine demethylase inhibitor. N-Oxalylglycine inhibits JMJD2A, JMJD2C and JMJD2D. N-Oxalylglycine also inhibits HIF prolylhydroxylase domain-1 (PHD-1) and PHD-2, with an IC50 of 2.1 μM and 5.6 μM, respectively.
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- Reinheit : 99.18%
- CAS. Nr.: 5262-39-5
- Formel: C4H5NO5
- Molecular Weight:147.09
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Speicherung:
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
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Biologische Aktivität
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Chemical Information
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Lösungsmittel & Löslichkeit
- Protokoll
- Reinheit & Dokumentation
- Verweise
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Help & FAQs
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Publications Citing Use of MedChemExpress (MCE) N-Oxalylglycine
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Biologische Aktivität
Beschreibung
IC50 & Target
IC50: 250 μM (JMJD2A) and 500 μM (JMJD2C)[2]
IC50: 2.1 μM (PHD-1) and 5.6 μM (PHD-2)[2]
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| HeLa | GI50 |
>710 μM
Compound: 1, NOG
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Growth inhibition of human HeLa cells after 48 hrs by MTT assay
Growth inhibition of human HeLa cells after 48 hrs by MTT assay
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[PMID: 23964788] |
| KYSE-150 cell line | GI50 |
>240 μM
Compound: 1, NOG
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Growth inhibition of human KYSE-150 cells after 48 hrs by MTT assay
Growth inhibition of human KYSE-150 cells after 48 hrs by MTT assay
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[PMID: 23964788] |
| Sf9 | IC50 |
2.1 μM
Compound: 2, NOG
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Inhibition of human recombinant PHD1 expressed in Sf9 cells by time-resolved fluorescence assay
Inhibition of human recombinant PHD1 expressed in Sf9 cells by time-resolved fluorescence assay
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[PMID: 20684604] |
| Sf9 | IC50 |
5.6 μM
Compound: 2, NOG
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Inhibition of human recombinant PHD2 expressed in Sf9 cells by time-resolved fluorescence assay
Inhibition of human recombinant PHD2 expressed in Sf9 cells by time-resolved fluorescence assay
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[PMID: 20684604] |
In Vitro
N-Oxalylglycine (1 mM) significantly curtails the demethylation activity of JMJD2A and JMJD2C in GST fusion proteins of the catalytic N-terminus of JMJD2A and 2C[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Chemical Information
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CAS. Nr. 5262-39-5
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Appearance Solid
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Molecular Weight 147.09
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Formel C4H5NO5
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Color White to off-white
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SMILES
O=C(O)CNC(C(O)=O)=O
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Synonyms
N-Oxaloglycine
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Versand
Room temperature in continental US; may vary elsewhere.
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Speicherung
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Publications (1)
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Journal Impact Factor
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Most Recent
Lösungsmittel & Löslichkeit
In Vitro:
H2O : 100 mg/mL (679.86 mM; Need ultrasonic)
DMSO : < 1 mg/mL (insoluble or slightly soluble)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Konzentration (Stammlösung) × Volumen (Stammlösung) = Konzentration (Ziellösung) × Volumen (Ziellösung)
Protokoll
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Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
Reinheit & Dokumentation
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Data Sheet (270 KB)
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SDS (761 KB)
- English - EN (761 KB)
- Français - FR (761 KB)
- Deutsch - DE (761 KB)
- Norwegian - NO (761 KB)
- Español - ES (761 KB)
- Swedish - SV (761 KB)
- Italian - IT (761 KB)
- Korean - KR (761 KB)
- Portuguese - PT (761 KB)
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Handling Instructions (2659 KB)
Verweise
[1]. Hamada S, et al. Synthesis and activity of N-oxalylglycine and its derivatives as Jumonji C-domain-containing histone lysine demethylase inhibitors. Bioorg Med Chem Lett. 2009 May 15;19(10):2852-5. [Content Brief]
[2]. Hamada S, et al. Design, synthesis, enzyme-inhibitory activity, and effect on human cancer cells of a novel series of jumonji domain-containing protein 2 histone demethylase inhibitors. J Med Chem. 2010 Aug 12;53(15):5629-38. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O | 1 mM | 6.7986 mL | 33.9928 mL | 67.9856 mL | 169.9640 mL |
| 5 mM | 1.3597 mL | 6.7986 mL | 13.5971 mL | 33.9928 mL | |
| 10 mM | 0.6799 mL | 3.3993 mL | 6.7986 mL | 16.9964 mL | |
| 15 mM | 0.4532 mL | 2.2662 mL | 4.5324 mL | 11.3309 mL | |
| 20 mM | 0.3399 mL | 1.6996 mL | 3.3993 mL | 8.4982 mL | |
| 25 mM | 0.2719 mL | 1.3597 mL | 2.7194 mL | 6.7986 mL | |
| 30 mM | 0.2266 mL | 1.1331 mL | 2.2662 mL | 5.6655 mL | |
| 40 mM | 0.1700 mL | 0.8498 mL | 1.6996 mL | 4.2491 mL | |
| 50 mM | 0.1360 mL | 0.6799 mL | 1.3597 mL | 3.3993 mL | |
| 60 mM | 0.1133 mL | 0.5665 mL | 1.1331 mL | 2.8327 mL | |
| 80 mM | 0.0850 mL | 0.4249 mL | 0.8498 mL | 2.1245 mL | |
| 100 mM | 0.0680 mL | 0.3399 mL | 0.6799 mL | 1.6996 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
N-Oxalylglycine Related Classifications
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