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NCT-504 is a selective allosteric inhibitor of PIP4Kγ, with an IC50 of 15.8 μM. NCT-504 is potential for the research of Huntington's disease.

For research use only. We do not sell to patients.

NCT-504 Chemical Structure

NCT-504 Chemical Structure

CAS No. : 1222765-97-0

Size Price Stock Quantity
Solid + Solvent
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 352 In-stock
Solution
10 mM * 1 mL in DMSO USD 352 In-stock
Solid
5 mg USD 320 In-stock
10 mg USD 510 In-stock
25 mg USD 1050 In-stock
50 mg USD 1680 In-stock
100 mg USD 2650 In-stock
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Customer Review

Based on 1 publication(s) in Google Scholar

NCT-504 Related Antibodies

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  • Biological Activity

  • Purity & Documentation

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Description

NCT-504 is a selective allosteric inhibitor of PIP4Kγ, with an IC50 of 15.8 μM. NCT-504 is potential for the research of Huntington's disease[1].

IC50 & Target

IC50: 15.8 μM (PIP4Kγ)[1]
In Vitro

NCT-504 dose not impair the intrinsic ATP-hydrolytic activity of PIP4Kγ in the absence of PI5P substrate[1].
NCT-504 does not inhibit PIP4Kbeta and weakly inhibits PIP4Kalpha phosphorylation of PI5P [1].
NCT-504 dose not inhibit PIP4Kbeta or PIP4Kalpha (IC50 between 50 μM and 100 μM) at 50 μM concentration[1].
NCT-504 elevates the levels of PI(3,5)P2, PI3P and PI5P in MEFs[1].
NCT-504 (10 μM; 12 hours) does not affect cell viability in MEFs[1].
NCT-504 (5 μM, 10 μM; 2 hours, 6 hours) elevates both the induction of autophagy as well as the rate of turnover of autophagic cargo[1].
NCT-504 treatment causes a robust increase in the formation of autolysosomes with only a modest elevation in autophagosomes[1].
NCT-504 increases autophagy flux and decreases huntingtin protein in 293A cells[1].
NCT-504 reduces mHtt protein levels in immortalized striatal cells from knock-in HD mice[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

NCT-504 Related Antibodies

Western Blot Analysis[1]

Cell Line: HEK293T cells
Concentration: 5 μM, 10 μM
Incubation Time: 2 hours, 6 hours
Result: Induced autophagosome formation.
Molecular Weight

404.49

Formula

C15H12N6O2S3
CAS No.
Appearance

Solid

Color

White to light yellow

SMILES

CN1N=NN=C1SC2=C(C(C3=CC=CC(S(=O)(C)=O)=C3)=CS4)C4=NC=N2
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 125 mg/mL (309.03 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.4722 mL 12.3612 mL 24.7225 mL
5 mM 0.4944 mL 2.4722 mL 4.9445 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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  • Dilution Calculator

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In Vivo Dissolution Calculator
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Working solution concentration: mg/mL
Purity & Documentation

Purity: 98.70%

COA (257 KB) HNMR (262 KB) LCMS (268 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.4722 mL 12.3612 mL 24.7225 mL 61.8062 mL
5 mM 0.4944 mL 2.4722 mL 4.9445 mL 12.3612 mL
10 mM 0.2472 mL 1.2361 mL 2.4722 mL 6.1806 mL
15 mM 0.1648 mL 0.8241 mL 1.6482 mL 4.1204 mL
20 mM 0.1236 mL 0.6181 mL 1.2361 mL 3.0903 mL
25 mM 0.0989 mL 0.4944 mL 0.9889 mL 2.4722 mL
30 mM 0.0824 mL 0.4120 mL 0.8241 mL 2.0602 mL
40 mM 0.0618 mL 0.3090 mL 0.6181 mL 1.5452 mL
50 mM 0.0494 mL 0.2472 mL 0.4944 mL 1.2361 mL
60 mM 0.0412 mL 0.2060 mL 0.4120 mL 1.0301 mL
80 mM 0.0309 mL 0.1545 mL 0.3090 mL 0.7726 mL
100 mM 0.0247 mL 0.1236 mL 0.2472 mL 0.6181 mL
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NCT-504 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

NCT-5041222765-97-0NCT504NCT 504OthersneurodegenerativedisordersHuntington'sdiseasemHttneurodegenerationInhibitorinhibitorinhibit

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