Cat. No.: HY-130246A

NF-56-EJ40 hydrochloride

14 Cited Publications

Customer Review

Based on 14 publication(s) in Google Scholar

NF-56-EJ40 hydrochloride is a potent, high-affinity, and highly selective human SUCNR1 (GPR91) antagonist with an IC₅₀ of 25 nM and a K_i of 33 nM, and shows almost no activity towards rat SUCNR1. NF-56-EJ40 hydrochloride has high affinity for humanized rat SUCNR1 with a K_i value of 17.4 nM.

For research use only. We do not sell to patients.

Purity: 98.45%
CAS No.: 2728500-80-7
Formula: C₂₇H₃₁Cl₂N₃O₃
Molecular Weight:516.46

Storage:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month

NF-56-EJ40 hydrochloride Chemical Structure

CAS No.: 2728500-80-7

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Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity

Description

Chemical Information

CAS No. 2728500-80-7
Appearance Solid
Molecular Weight 516.46
Formula C₂₇H₃₁Cl₂N₃O₃
Color Off-white to light brown
SMILES

O=C(NC1=C(CC(O)=O)C=CC=C1)C2=CC=CC(C3=CC=C(CN4CCN(C)CC4)C=C3)=C2.Cl.Cl
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Publications (14)

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Metabolism

Protective effects of the succinate/SUCNR1 axis on damaged hepatocytes in NAFLD. [Abstract]

2023 Aug:145:155630. PMID: 37315889
J Clin Invest

Hyperglycemia aggravates vitiligo through succinate/SUCNR1-mediated T cell activation. [Abstract]

2026 Jun 15;136(12):e200316. PMID: 42294897

NF-56-EJ40 hydrochloride purchased from MedChemExpress. Usage Cited in: J Clin Invest. 2026 Jun 15;136(12):e200316. [Abstract]

NF-56-EJ40 (50 nM; 3 h) markedly dampened succinate-induced increase of CXCL9 and CXCL10 mRNA in Normal human keratinocytes (NHKs).

NF-56-EJ40 hydrochloride purchased from MedChemExpress. Usage Cited in: J Clin Invest. 2026 Jun 15;136(12):e200316. [Abstract]

NF-56-EJ40 (50 nM; 3 h) markedly dampened succinate-induced increase of CXCL9 and CXCL10 protein in Normal human keratinocytes (NHKs).

NF-56-EJ40 hydrochloride purchased from MedChemExpress. Usage Cited in: J Clin Invest. 2026 Jun 15;136(12):e200316. [Abstract]

NF-56-EJ40 (50 nM; 3 h) reversed succinate-induced HIF-1α upregulation in Normal human keratinocytes (NHKs).

NF-56-EJ40 hydrochloride purchased from MedChemExpress. Usage Cited in: J Clin Invest. 2026 Jun 15;136(12):e200316. [Abstract]

NF-56-EJ40 (50 nM; 3 h) decreased HIF-1α expression in the cell nucleus in Normal human keratinocytes (NHKs).

NF-56-EJ40 hydrochloride purchased from MedChemExpress. Usage Cited in: J Clin Invest. 2026 Jun 15;136(12):e200316. [Abstract]

NF-56-EJ40 (50 nM; 3 h) inhibited the succinate-induced nuclear translocation of HIF-1α in Normal human keratinocytes (NHKs).
J Clin Invest

SUCNR1 regulates insulin secretion and glucose elevates the succinate response in people with prediabetes. [Abstract]

2024 May 7:e173214. PMID: 38713514

NF-56-EJ40 hydrochloride purchased from MedChemExpress. Usage Cited in: J Clin Invest. 2024 May 7:e173214. [Abstract]

NF-56-EJ40 (1 μM; 3 h) completely blocked succinate-induced insulin secretion in the presence of high glucose in EndoC-βH5 cells.
Sci Adv

SUCNR1 coordinates metabolic flux, mitochondrial function, and nutrient-dependent adaptation in hepatocytes. [Abstract]

2026 Jun 12;12(24):eaec8873. PMID: 42284407
Cardiovasc Res

miR-369-3p ameliorates diabetes-associated atherosclerosis by regulating macrophage succinate-GPR91 signaling. [Abstract]

2024 Nov 25;120(14):1693-1712. PMID: 38703377

NF-56-EJ40 hydrochloride purchased from MedChemExpress. Usage Cited in: Cardiovasc Res. 2024 Nov 25;120(14):1693-1712. [Abstract]

NF-56-EJ40 (5 μM; 30 min+24 h) significantly reduced the OxLDL-induced IL-1β secretion in healthy and diabetic monocytes compared to control.
Cancer Lett

Succinate receptor 1 inhibits mitochondrial respiration in cancer cells addicted to glutamine. [Abstract]

2022 Feb 1:526:91-102. PMID: 34813893
Dev Cell

Pulmonary neuroendocrine cells sense succinate to stimulate myoepithelial cell contraction. [Abstract]

2022 Sep 26;57(18):2221-2236.e5. PMID: 36108628
Arch Pharm Res

Silybin inhibits succinate production and secretion in hepatocytes to reverse liver fibrosis. [Abstract]

2025 Aug;48(7-8):782-797. PMID: 40739373
J Agric Food Chem

Exposure to Succinate Leads to Steatosis in Non-Obese Non-Alcoholic Fatty Liver Disease by Inhibiting AMPK/PPARα/FGF21-Dependent Fatty Acid Oxidation. [Abstract]

2024 Sep 25;72(38):21052-21064. PMID: 39268842
Cell Signal

GPR91 mediates low shear stress-induced endothelial ferroptosis via EGR1-dependent GPX4 repression. [Abstract]

2025 Sep 10:136:112127. PMID: 40939914
Oncol Res

SDHA Deficiency in Hepatocellular Carcinoma Promotes Tumor Progression through Succinate-Induced M2 Macrophage Polarization. [Abstract]

2026 Jan 19;34(2):25. PMID: 41613800
J Physiol

Impaired angiogenesis in gestational diabetes is linked to succinate/SUCNR1 axis dysregulation in late gestation. [Abstract]

2025 Mar 31. PMID: 40163642
Poult Sci

Lactobacillus salivarius metabolite succinate enhances chicken intestinal stem cell activities via the SUCNR1-mitochondria axis. [Abstract]

2024 Dec 31;104(2):104754. PMID: 39764876
bioRxiv

January 10, 2022.

Solvent & Solubility

In Vitro:

DMSO : 5.19 mg/mL (10.05 mM; Need ultrasonic and warming; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

1.9363 mL

9.6813 mL

19.3626 mL

5 mM

0.3873 mL

1.9363 mL

3.8725 mL

10 mM

0.1936 mL

0.9681 mL

1.9363 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (273 KB)
SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Haffke M, et al. Structural basis of species-selective antagonist binding to the succinate receptor. Nature. 2019 Oct;574(7779):581-585. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	1.9363 mL	9.6813 mL	19.3626 mL	48.4065 mL
	5 mM	0.3873 mL	1.9363 mL	3.8725 mL	9.6813 mL
	10 mM	0.1936 mL	0.9681 mL	1.9363 mL	4.8406 mL

Keywords

NF-56-EJ40 hydrochloride

14 Cited Publications

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NF-56-EJ40 hydrochloride

GPCR/G Protein Compound Library

Immunology/Inflammation Compound Library

Membrane Protein-targeted Compound Library

Membrane Receptor-targeted Compound Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Bioactive Compound Library

High-Throughput Bioactive Compound Library

Lactylation Compound Library

Publications Citing Use of MedChemExpress (MCE) NF-56-EJ40 hydrochloride

Biological Activity

Chemical Information

Publications (14)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

NF-56-EJ40 hydrochloride

GPCR/G Protein Compound Library

Immunology/Inflammation Compound Library

Membrane Protein-targeted Compound Library

Membrane Receptor-targeted Compound Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Bioactive Compound Library

High-Throughput Bioactive Compound Library

Lactylation Compound Library

Publications Citing Use of MedChemExpress (MCE) NF-56-EJ40 hydrochloride

Biological Activity

Chemical Information

Publications (14)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

NF-56-EJ40 hydrochloride Related Classifications

HY-130246A Related Classifications