Cat. No.: HY-130246A

NF-56-EJ40 hydrochloride

Name: NF-56-EJ40 hydrochloride
Brand: MedChemExpress
SKU: HY-130246A
Price: 58.00 USD
Availability: InStock
Rating: 5 (16 reviews)

16 Cited Publications

Customer Review

Based on 16 publication(s) in Google Scholar

NF-56-EJ40 hydrochloride is a potent, high-affinity, and highly selective human SUCNR1 (GPR91) antagonist with an IC₅₀ of 25 nM and a K_i of 33 nM, and shows almost no activity towards rat SUCNR1. NF-56-EJ40 hydrochloride has high affinity for humanized rat SUCNR1 with a K_i value of 17.4 nM.

For research use only. We do not sell to patients.

Purity : 99.94%
CAS No.: 2728500-80-7
Formula: C₂₇H₃₁Cl₂N₃O₃
Molecular Weight:516.46

Storage:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month

NF-56-EJ40 hydrochloride Chemical Structure

CAS No.: 2728500-80-7

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Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

HY-130246
NF-56-EJ40

SUCNR1 (GPR91) Antagonist

Biological Activity
- Description
Chemical Information
Publications (16)
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

Chemical Information

CAS No. 2728500-80-7
Appearance Solid
Molecular Weight 516.46
Formula C₂₇H₃₁Cl₂N₃O₃
Color Off-white to light brown
SMILES

O=C(NC1=C(CC(O)=O)C=CC=C1)C2=CC=CC(C3=CC=C(CN4CCN(C)CC4)C=C3)=C2.Cl.Cl
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Publications (16)

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Journal Impact Factor

Metabolism

Protective effects of the succinate/SUCNR1 axis on damaged hepatocytes in NAFLD. [Abstract]

2023 Aug:145:155630. PMID: 37315889
J Clin Invest

Hyperglycemia aggravates vitiligo through succinate/SUCNR1-mediated T cell activation. [Abstract]

2026 Jun 15;136(12):e200316. PMID: 42294897

NF-56-EJ40 hydrochloride purchased from MedChemExpress. Usage Cited in: J Clin Invest. 2026 Jun 15;136(12):e200316. [Abstract]

NF-56-EJ40 (50 nM; 3 h) markedly dampened succinate-induced increase of CXCL9 and CXCL10 mRNA in Normal human keratinocytes (NHKs).

NF-56-EJ40 hydrochloride purchased from MedChemExpress. Usage Cited in: J Clin Invest. 2026 Jun 15;136(12):e200316. [Abstract]

NF-56-EJ40 (50 nM; 3 h) markedly dampened succinate-induced increase of CXCL9 and CXCL10 protein in Normal human keratinocytes (NHKs).

NF-56-EJ40 hydrochloride purchased from MedChemExpress. Usage Cited in: J Clin Invest. 2026 Jun 15;136(12):e200316. [Abstract]

NF-56-EJ40 (50 nM; 3 h) reversed succinate-induced HIF-1α upregulation in Normal human keratinocytes (NHKs).

NF-56-EJ40 hydrochloride purchased from MedChemExpress. Usage Cited in: J Clin Invest. 2026 Jun 15;136(12):e200316. [Abstract]

NF-56-EJ40 (50 nM; 3 h) decreased HIF-1α expression in the cell nucleus in Normal human keratinocytes (NHKs).

NF-56-EJ40 hydrochloride purchased from MedChemExpress. Usage Cited in: J Clin Invest. 2026 Jun 15;136(12):e200316. [Abstract]

NF-56-EJ40 (50 nM; 3 h) inhibited the succinate-induced nuclear translocation of HIF-1α in Normal human keratinocytes (NHKs).
J Clin Invest

SUCNR1 regulates insulin secretion and glucose elevates the succinate response in people with prediabetes. [Abstract]

2024 May 7;134(12):e173214. PMID: 38713514

NF-56-EJ40 hydrochloride purchased from MedChemExpress. Usage Cited in: J Clin Invest. 2024 May 7;134(12):e173214. [Abstract]

NF-56-EJ40 (1 μM; 3 h) completely blocked succinate-induced insulin secretion in the presence of high glucose in EndoC-βH5 cells.
Sci Adv

SUCNR1 coordinates metabolic flux, mitochondrial function, and nutrient-dependent adaptation in hepatocytes. [Abstract]

2026 Jun 12;12(24):eaec8873. PMID: 42284407
Cardiovasc Res

miR-369-3p ameliorates diabetes-associated atherosclerosis by regulating macrophage succinate-GPR91 signaling. [Abstract]

2024 Nov 25;120(14):1693-1712. PMID: 38703377

NF-56-EJ40 hydrochloride purchased from MedChemExpress. Usage Cited in: Cardiovasc Res. 2024 Nov 25;120(14):1693-1712. [Abstract]

NF-56-EJ40 (5 μM; 30 min+24 h) significantly reduced the OxLDL-induced IL-1β secretion in healthy and diabetic monocytes compared to control.
Cancer Lett

Succinate receptor 1 inhibits mitochondrial respiration in cancer cells addicted to glutamine. [Abstract]

2022 Feb 1:526:91-102. PMID: 34813893
Dev Cell

Pulmonary neuroendocrine cells sense succinate to stimulate myoepithelial cell contraction. [Abstract]

2022 Sep 26;57(18):2221-2236.e5. PMID: 36108628
Arch Pharm Res

Silybin inhibits succinate production and secretion in hepatocytes to reverse liver fibrosis. [Abstract]

2025 Aug;48(7-8):782-797. PMID: 40739373
J Agric Food Chem

Exposure to Succinate Leads to Steatosis in Non-Obese Non-Alcoholic Fatty Liver Disease by Inhibiting AMPK/PPARα/FGF21-Dependent Fatty Acid Oxidation. [Abstract]

2024 Sep 25;72(38):21052-21064. PMID: 39268842
Int Immunopharmacol

2026 Oct 15:187:117198. PMID: 42537347
Int J Mol Sci

2026 Sep 5;27(17):7930. PMID: 42737827
Cell Signal

GPR91 mediates low shear stress-induced endothelial ferroptosis via EGR1-dependent GPX4 repression. [Abstract]

2025 Sep 10:136:112127. PMID: 40939914
Oncol Res

SDHA Deficiency in Hepatocellular Carcinoma Promotes Tumor Progression through Succinate-Induced M2 Macrophage Polarization. [Abstract]

2026 Jan 19;34(2):25. PMID: 41613800
J Physiol

Impaired angiogenesis in gestational diabetes is linked to succinate/SUCNR1 axis dysregulation in late gestation. [Abstract]

2025 Mar 31. PMID: 40163642
Poult Sci

Lactobacillus salivarius metabolite succinate enhances chicken intestinal stem cell activities via the SUCNR1-mitochondria axis. [Abstract]

2024 Dec 31;104(2):104754. PMID: 39764876
bioRxiv

January 10, 2022.

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (193.63 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

1.9363 mL

9.6813 mL

19.3626 mL

5 mM

0.3873 mL

1.9363 mL

3.8725 mL

10 mM

0.1936 mL

0.9681 mL

1.9363 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (4.84 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (4.84 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (275 KB)
SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Haffke M, et al. Structural basis of species-selective antagonist binding to the succinate receptor. Nature. 2019 Oct;574(7779):581-585. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	1.9363 mL	9.6813 mL	19.3626 mL	48.4065 mL
	5 mM	0.3873 mL	1.9363 mL	3.8725 mL	9.6813 mL
	10 mM	0.1936 mL	0.9681 mL	1.9363 mL	4.8406 mL
	15 mM	0.1291 mL	0.6454 mL	1.2908 mL	3.2271 mL
	20 mM	0.0968 mL	0.4841 mL	0.9681 mL	2.4203 mL
	25 mM	0.0775 mL	0.3873 mL	0.7745 mL	1.9363 mL
	30 mM	0.0645 mL	0.3227 mL	0.6454 mL	1.6135 mL
	40 mM	0.0484 mL	0.2420 mL	0.4841 mL	1.2102 mL
	50 mM	0.0387 mL	0.1936 mL	0.3873 mL	0.9681 mL
	60 mM	0.0323 mL	0.1614 mL	0.3227 mL	0.8068 mL
	80 mM	0.0242 mL	0.1210 mL	0.2420 mL	0.6051 mL
	100 mM	0.0194 mL	0.0968 mL	0.1936 mL	0.4841 mL

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NF-56-EJ40 hydrochloride

16 Cited Publications

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NF-56-EJ40 hydrochloride

Publications Citing Use of MedChemExpress (MCE) NF-56-EJ40 hydrochloride