Cat. No.: HY-144765

NF-κB-IN-4

1 Cited Publications

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Based on 1 publication(s) in Google Scholar

NF-κB-IN-4 (compound 17) is a potent NF-κB pathway inhibitor with blood brain barrier (BBB) permeability. NF-κB-IN-4 exhibits potential anti-neuroinflammatory activity with low toxicity. NF-κB-IN-4 can block the activation and phosphorylation of IκBα, reduce expression of NLRP3, and thus inhibit NF-κB activation. NF-κB-IN-4 can be used for neuroinflammation related diseases research.

For research use only. We do not sell to patients.

Purity: 98.51%
CAS No.: 3033258-60-2
Formula: C₁₈H₁₅FN₄O
Molecular Weight:322.34

Storage:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month

NF-κB-IN-4 Chemical Structure

CAS No.: 3033258-60-2

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Biological Activity
- Description
- In Vitro
Chemical Information
Publications (1)
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

All NF-κB Isoforms :

In Vitro

NF-κB-IN-4 (compound 17) (0-10 μM, 24 h) shows high anti-inflammatory activity^[1].
NF-κB-IN-4 (0-5 μM, 24 h) significantly decreases the LPS-induced p-IκBα expression levels, significantly inhibits the phosphorylation of IκBα and the expression of three proteins (NLRP3, ASC and caspase-1) in BV2 cells^[1].
NF-κB-IN-4 (0-5 μM, 24 h) induces LPS-induced apoptosis in BV2 cells in a dose-dependent manner^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Cytotoxicity Assay

Cell Line:

BV2 microglia cells^[1]
Concentration:

0, 2.5, 5, 10 μM
Incubation Time:

24 h
Result:

Showed high anti-inflammatory activity, with the survival rate of BV2 microglia cells of 97.4%, inhibition rates against TNF-α and IL-6 release of 60.8% and 80.2%, respectively.

Western Blot Analysis

Cell Line:

BV2 cells^[1]
Concentration:

0, 1.25, 2.5, 5 μM
Incubation Time:

24 h
Result:

Significantly decreased the LPS-induced p-IκBα expression levels, significantly inhibited the phosphorylation of IκBα and the expression of three proteins (NLRP3, ASC and caspase-1) in BV2 cells.

Apoptosis Analysis

Cell Line:

BV2 cells^[1]
Concentration:

0, 2.5, 5 μM
Incubation Time:

24 h
Result:

Induced LPS-induced apoptosis in BV2 cells in a dose-dependent manner, with the apoptosis rates of 30.7% (2.5 μM) and 13.0% (5 μM), respectively.

Chemical Information

CAS No. 3033258-60-2
Appearance Solid
Molecular Weight 322.34
Formula C₁₈H₁₅FN₄O
Color Off-white to light yellow
SMILES

COC1=CC(C2=C3CCC4=C(C3=NC(N)=N2)C=C(C=C4)F)=CN=C1
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Publications (1)

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SSRN

2025 Jan 23.

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (310.23 mM; ultrasonic and warming and heat to 150°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

3.1023 mL

15.5116 mL

31.0231 mL

5 mM

0.6205 mL

3.1023 mL

6.2046 mL

10 mM

0.3102 mL

1.5512 mL

3.1023 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

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In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (7.76 mM); Suspended solution

This protocol yields a suspended solution of ≥ 2.5 mg/mL (saturation unknown). Suspended solution can be used for oral and intraperitoneal injection.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (275 KB)
SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Zhang XF, et al. Anti-neuroinflammatory effects of novel 5,6-dihydrobenzo[h]quinazolin-2-amine derivatives in lipopolysaccharide-stimulated BV2 microglial cells. Eur J Med Chem. 2022;235:114322. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	3.1023 mL	15.5116 mL	31.0231 mL	77.5579 mL
	5 mM	0.6205 mL	3.1023 mL	6.2046 mL	15.5116 mL
	10 mM	0.3102 mL	1.5512 mL	3.1023 mL	7.7558 mL
	15 mM	0.2068 mL	1.0341 mL	2.0682 mL	5.1705 mL
	20 mM	0.1551 mL	0.7756 mL	1.5512 mL	3.8779 mL
	25 mM	0.1241 mL	0.6205 mL	1.2409 mL	3.1023 mL
	30 mM	0.1034 mL	0.5171 mL	1.0341 mL	2.5853 mL
	40 mM	0.0776 mL	0.3878 mL	0.7756 mL	1.9389 mL
	50 mM	0.0620 mL	0.3102 mL	0.6205 mL	1.5512 mL
	60 mM	0.0517 mL	0.2585 mL	0.5171 mL	1.2926 mL
	80 mM	0.0388 mL	0.1939 mL	0.3878 mL	0.9695 mL
	100 mM	0.0310 mL	0.1551 mL	0.3102 mL	0.7756 mL

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NF-κB-IN-4

Apoptosis Compound Library

Immunology/Inflammation Compound Library

NF-κB Signaling Compound Library

Stem Cell Signaling Compound Library

Anti-Cancer Compound Library

Anti-Aging Compound Library

Antioxidant Compound Library

Differentiation Inducing Compound Library

Oxygen Sensing Compound Library

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Cell Death Library

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Anti-Ovarian Cancer Compound Library

Inflammasomes related Compound Library

Radioprotector Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

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Non-Alcoholic Fatty Liver Disease (NAFLD) Compound Library

Cell Proliferation Compound Library

High-Throughput Bioactive Compound Library

Lactylation Compound Library

Publications Citing Use of MedChemExpress (MCE) NF-κB-IN-4

All NF-κB Isoforms

Biological Activity

Chemical Information

Publications (1)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

NF-κB-IN-4 Related Classifications

HY-144765 Related Classifications