NG-nitro-L-arginine (Synonyms: Nω-nitro-L-arginine; L-NOARG; L-NG-Nitroarginine)

Cat. No.: HY-12115 Purity: 99.78%: Data Sheet
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NG-nitro-L-arginine (Nω-nitro-L-arginine) is a NO Synthase inhibitor, with K_is of 0.61 μM (nNOS), 4.28 μM (iNOS), 0.72 μM (eNOS) respectively. NG-nitro-L-arginine inhibits endothelium-derived relaxing factor (EDRF) formation and release. NG-nitro-L-arginine inhibits portal-systemic shunting in portal-hypertensive rats, and increases blood pressure.

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NG-nitro-L-arginine Chemical Structure

CAS No. : 2149-70-4

Size	Stock	Quantity
25 g	In-stock	Estimated Time of Arrival: December 31
50 g	In-stock	Estimated Time of Arrival: December 31
100 g	In-stock	Estimated Time of Arrival: December 31
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nNOS iNOS eNOS

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Purity & Documentation
References
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Description

IC₅₀ & Target

K_is: 0.61 μM (nNOS), 4.28 μM (iNOS), 0.72 μM (eNOS)^[1]

Cellular Effect

Cell Line	Type	Value	Description	References
HEK-293T	IC₅₀	5 μM Compound: L-NNA	Inhibition of rat nNOS expressed in HEK293T cells preincubated for 30 mins prior A23187-induced activation measured after 6 hrs by Griess assay Inhibition of rat nNOS expressed in HEK293T cells preincubated for 30 mins prior A23187-induced activation measured after 6 hrs by Griess assay	[PMID: 22370337]
RAW	IC₅₀	14 μM Compound: 1	Inhibition of radiolabeled arginine conversion to citrulline by isomeric form of Inducible nitric oxide synthase from mouse RAW 264.7 cells Inhibition of radiolabeled arginine conversion to citrulline by isomeric form of Inducible nitric oxide synthase from mouse RAW 264.7 cells	[PMID: 9651169]
RAW264.7	CC₅₀	> 100 μM Compound: Nomega-nitro-L-arginine	Cytotoxicity against mouse RAW264.7 cells assessed as reduction in cell viability after 3 hrs by alamar blue assay Cytotoxicity against mouse RAW264.7 cells assessed as reduction in cell viability after 3 hrs by alamar blue assay	[PMID: 22148193]
RAW264.7	IC₅₀	143.55 μM Compound: Nomega-nitro-L-arginine	Inhibition of iNOS-mediated NO production in LPS-stimulated mouse RAW264.7 cells after 24 hrs by Griess reagent method Inhibition of iNOS-mediated NO production in LPS-stimulated mouse RAW264.7 cells after 24 hrs by Griess reagent method	[PMID: 22148193]
RAW264.7	IC₅₀	144.3 μM Compound: N-Omega-nitro-L-arginine	Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-stimulated nitric oxide production compound treated prior to LPS challenge by Griess assay Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-stimulated nitric oxide production compound treated prior to LPS challenge by Griess assay	[PMID: 27976895]
RAW264.7	IC₅₀	146.4 μM Compound: L-NNA	Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced nitric oxide production by spectrophotometric analysis Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced nitric oxide production by spectrophotometric analysis	[PMID: 24597849]
RAW264.7	IC₅₀	152.46 μM Compound: N-omega-nitro-L-arginine	Inhibition of iNOS in mouse RAW264.7 cells assessed as anti-inflammatory activity by measuring maximum inhibition of LPS-induced nitric oxide production after 24 hrs Inhibition of iNOS in mouse RAW264.7 cells assessed as anti-inflammatory activity by measuring maximum inhibition of LPS-induced nitric oxide production after 24 hrs	[PMID: 21846089]
RAW264.7	IC₅₀	2.918 μM Compound: L-NNA	Anti-inflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced nitric oxide synthase production measured after 24 hrs by ELISA Anti-inflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced nitric oxide synthase production measured after 24 hrs by ELISA	[PMID: 33065432]
RAW264.7	IC₅₀	37.7 μM Compound: L-NA	Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced nitric oxide production by measuring nitrite accumulation by Griess method Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced nitric oxide production by measuring nitrite accumulation by Griess method	[PMID: 28561586]
RAW264.7	IC₅₀	39.5 μM Compound: N-nitro-L-arginine	Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced NO production preincubated for 1 hrs followed by LPS stimulation and measured after 24 hrs by Griess reagent based spectrophotometrical method Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced NO production preincubated for 1 hrs followed by LPS stimulation and measured after 24 hrs by Griess reagent based spectrophotometrical method	[PMID: 33422907]
RAW264.7	IC₅₀	43.6 μM Compound: L-NA	Antiinflammatory activity against mouse RAW264.7 cells assessed as inhibition of LPS-induced nitric oxide production after 24 hrs by Griess reagent method Antiinflammatory activity against mouse RAW264.7 cells assessed as inhibition of LPS-induced nitric oxide production after 24 hrs by Griess reagent method	[PMID: 19715321]

Clinical Trial

Molecular Weight

219.20

Formula

C₆H₁₃N₅O₄

CAS No.

2149-70-4

Appearance

Solid

Color

White to off-white

SMILES

N[C@@H](CCCNC(N[N+]([O-])=O)=N)C(O)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Store at room temperature 3 years

In solvent	-80°C	2 years
	-20°C	1 year

Solvent & Solubility

In Vitro:

1 M HCl : 100 mg/mL (456.20 mM; Need ultrasonic)

H₂O : 3.33 mg/mL (15.19 mM; ultrasonic and adjust pH to 2 with HCl)

DMSO : < 1 mg/mL (insoluble or slightly soluble)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	4.5620 mL	22.8102 mL	45.6204 mL
5 mM	0.9124 mL	4.5620 mL	9.1241 mL
10 mM	0.4562 mL	2.2810 mL	4.5620 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

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Purity & Documentation

Purity: 99.78%

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Data Sheet (270 KB) SDS (604 KB)

COA (244 KB) HNMR (251 KB) LCMS (88 KB)

Handling Instructions (2659 KB)

References

[1]. Seo J, et al. Structure-based design and synthesis of N(omega)-nitro-L-arginine-containing peptidomimetics as selective inhibitors of neuronal nitric oxide synthase. Displacement of the heme structural water. J Med Chem. 2007 May 3;50(9):2089-99. [Content Brief]

[2]. Ishii K, et al. N omega-nitro-L-arginine: a potent inhibitor of endothelium-derived relaxing factor formation. Eur J Pharmacol. 1990 Feb 6;176(2):219-23. [Content Brief]

[3]. Lee FY, et al. Administration of N omega-nitro-L-arginine ameliorates portal-systemic shunting in portal-hypertensive rats. Gastroenterology. 1993 Nov;105(5):1464-70. [Content Brief]

[4]. Molnár M, Hertelendy F. N omega-nitro-L-arginine, an inhibitor of nitric oxide synthesis, increases blood pressure in rats and reverses the pregnancy-induced refractoriness to vasopressor agents. Am J Obstet Gynecol. 1992 May;166(5):1560-7. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O / 1 M HCl	1 mM	4.5620 mL	22.8102 mL	45.6204 mL	114.0511 mL
	5 mM	0.9124 mL	4.5620 mL	9.1241 mL	22.8102 mL
	10 mM	0.4562 mL	2.2810 mL	4.5620 mL	11.4051 mL
	15 mM	0.3041 mL	1.5207 mL	3.0414 mL	7.6034 mL
1 M HCl	20 mM	0.2281 mL	1.1405 mL	2.2810 mL	5.7026 mL
	25 mM	0.1825 mL	0.9124 mL	1.8248 mL	4.5620 mL
	30 mM	0.1521 mL	0.7603 mL	1.5207 mL	3.8017 mL
	40 mM	0.1141 mL	0.5703 mL	1.1405 mL	2.8513 mL
	50 mM	0.0912 mL	0.4562 mL	0.9124 mL	2.2810 mL
	60 mM	0.0760 mL	0.3802 mL	0.7603 mL	1.9009 mL
	80 mM	0.0570 mL	0.2851 mL	0.5703 mL	1.4256 mL
	100 mM	0.0456 mL	0.2281 mL	0.4562 mL	1.1405 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

NG-nitro-L-arginine Related Classifications

Cardiovascular Disease

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

NG-nitro-L-arginine2149-70-4Nω-nitro-L-arginine L-NOARG L-NG-NitroarginineNO SynthaseNitric oxide synthasesNOShypertensiveInhibitorinhibitorinhibit

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NG-nitro-L-arginine (Synonyms: Nω-nitro-L-arginine; L-NOARG; L-NG-Nitroarginine)

NG-nitro-L-arginine Related Antibodies

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Biological Activity

Purity & Documentation

References

Customer Review

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