NG-nitro-L-arginine
Based on 1 Customer Validation
NG-nitro-L-arginine (Nω-nitro-L-arginine) is a NO Synthase inhibitor, with Kis of 0.61 μM (nNOS), 4.28 μM (iNOS), 0.72 μM (eNOS) respectively. NG-nitro-L-arginine inhibits endothelium-derived relaxing factor (EDRF) formation and release. NG-nitro-L-arginine inhibits portal-systemic shunting in portal-hypertensive rats, and increases blood pressure.
For research use only. We do not sell to patients.
- Purity: 99.78%
- CAS No.: 2149-70-4
- Formula: C6H13N5O4
- Molecular Weight:219.20
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Storage:
Store at room temperature 3 years.
In solvent -80°C, 2 years , -20°C, 1 year
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Kis: 0.61 μM (nNOS), 4.28 μM (iNOS), 0.72 μM (eNOS)[1]
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| HEK-293T | IC50 |
5 μM
Compound: L-NNA
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Inhibition of rat nNOS expressed in HEK293T cells preincubated for 30 mins prior A23187-induced activation measured after 6 hrs by Griess assay
Inhibition of rat nNOS expressed in HEK293T cells preincubated for 30 mins prior A23187-induced activation measured after 6 hrs by Griess assay
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[PMID: 22370337] |
| RAW | IC50 |
14 μM
Compound: 1
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Inhibition of radiolabeled arginine conversion to citrulline by isomeric form of Inducible nitric oxide synthase from mouse RAW 264.7 cells
Inhibition of radiolabeled arginine conversion to citrulline by isomeric form of Inducible nitric oxide synthase from mouse RAW 264.7 cells
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[PMID: 9651169] |
| RAW264.7 | CC50 |
>100 μM
Compound: Nomega-nitro-L-arginine
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Cytotoxicity against mouse RAW264.7 cells assessed as reduction in cell viability after 3 hrs by alamar blue assay
Cytotoxicity against mouse RAW264.7 cells assessed as reduction in cell viability after 3 hrs by alamar blue assay
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[PMID: 22148193] |
| RAW264.7 | IC50 |
143.55 μM
Compound: Nomega-nitro-L-arginine
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Inhibition of iNOS-mediated NO production in LPS-stimulated mouse RAW264.7 cells after 24 hrs by Griess reagent method
Inhibition of iNOS-mediated NO production in LPS-stimulated mouse RAW264.7 cells after 24 hrs by Griess reagent method
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[PMID: 22148193] |
| RAW264.7 | IC50 |
144.3 μM
Compound: N-Omega-nitro-L-arginine
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Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-stimulated nitric oxide production compound treated prior to LPS challenge by Griess assay
Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-stimulated nitric oxide production compound treated prior to LPS challenge by Griess assay
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[PMID: 27976895] |
| RAW264.7 | IC50 |
146.4 μM
Compound: L-NNA
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Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced nitric oxide production by spectrophotometric analysis
Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced nitric oxide production by spectrophotometric analysis
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[PMID: 24597849] |
| RAW264.7 | IC50 |
152.46 μM
Compound: N-omega-nitro-L-arginine
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Inhibition of iNOS in mouse RAW264.7 cells assessed as anti-inflammatory activity by measuring maximum inhibition of LPS-induced nitric oxide production after 24 hrs
Inhibition of iNOS in mouse RAW264.7 cells assessed as anti-inflammatory activity by measuring maximum inhibition of LPS-induced nitric oxide production after 24 hrs
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[PMID: 21846089] |
| RAW264.7 | IC50 |
2.918 μM
Compound: L-NNA
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Anti-inflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced nitric oxide synthase production measured after 24 hrs by ELISA
Anti-inflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced nitric oxide synthase production measured after 24 hrs by ELISA
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[PMID: 33065432] |
| RAW264.7 | IC50 |
37.7 μM
Compound: L-NA
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Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced nitric oxide production by measuring nitrite accumulation by Griess method
Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced nitric oxide production by measuring nitrite accumulation by Griess method
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[PMID: 28561586] |
| RAW264.7 | IC50 |
39.5 μM
Compound: N-nitro-L-arginine
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Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced NO production preincubated for 1 hrs followed by LPS stimulation and measured after 24 hrs by Griess reagent based spectrophotometrical method
Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced NO production preincubated for 1 hrs followed by LPS stimulation and measured after 24 hrs by Griess reagent based spectrophotometrical method
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[PMID: 33422907] |
| RAW264.7 | IC50 |
43.6 μM
Compound: L-NA
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Antiinflammatory activity against mouse RAW264.7 cells assessed as inhibition of LPS-induced nitric oxide production after 24 hrs by Griess reagent method
Antiinflammatory activity against mouse RAW264.7 cells assessed as inhibition of LPS-induced nitric oxide production after 24 hrs by Griess reagent method
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[PMID: 19715321] |
| NCT Number | Sponsor | Condition | Start Date |
Phase
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|---|---|---|---|---|
| NCT01329991 | Plexxikon| | 2011-05 | PHASE1 |
Chemical Information
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CAS No. 2149-70-4
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Appearance Solid
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Molecular Weight 219.20
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Formula C6H13N5O4
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Color White to off-white
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SMILES
N[C@@H](CCCNC(N[N+]([O-])=O)=N)C(O)=O
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Synonyms
Nω-nitro-L-arginine; L-NOARG; L-NG-Nitroarginine
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Store at room temperature 3 years
In solvent -80°C 2 years -20°C 1 year
Solvent & Solubility
1 M HCl : 100 mg/mL (456.20 mM; Need ultrasonic)
H2O : 3.33 mg/mL (15.19 mM; ultrasonic and adjust pH to 2 with HCl)
DMSO : < 1 mg/mL (insoluble or slightly soluble)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (270 KB)
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SDS (604 KB)
- English - EN (604 KB)
- Français - FR (604 KB)
- Deutsch - DE (604 KB)
- Norwegian - NO (604 KB)
- Español - ES (604 KB)
- Swedish - SV (604 KB)
- Italian - IT (604 KB)
- Korean - KR (604 KB)
- Portuguese - PT (604 KB)
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Handling Instructions (2659 KB)
References
[1]. Seo J, et al. Structure-based design and synthesis of N(omega)-nitro-L-arginine-containing peptidomimetics as selective inhibitors of neuronal nitric oxide synthase. Displacement of the heme structural water. J Med Chem. 2007 May 3;50(9):2089-99. [Content Brief]
[2]. Ishii K, et al. N omega-nitro-L-arginine: a potent inhibitor of endothelium-derived relaxing factor formation. Eur J Pharmacol. 1990 Feb 6;176(2):219-23. [Content Brief]
[3]. Lee FY, et al. Administration of N omega-nitro-L-arginine ameliorates portal-systemic shunting in portal-hypertensive rats. Gastroenterology. 1993 Nov;105(5):1464-70. [Content Brief]
[4]. Molnár M, Hertelendy F. N omega-nitro-L-arginine, an inhibitor of nitric oxide synthesis, increases blood pressure in rats and reverses the pregnancy-induced refractoriness to vasopressor agents. Am J Obstet Gynecol. 1992 May;166(5):1560-7. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O / 1 M HCl | 1 mM | 4.5620 mL | 22.8102 mL | 45.6204 mL | 114.0511 mL |
| 5 mM | 0.9124 mL | 4.5620 mL | 9.1241 mL | 22.8102 mL | |
| 10 mM | 0.4562 mL | 2.2810 mL | 4.5620 mL | 11.4051 mL | |
| 15 mM | 0.3041 mL | 1.5207 mL | 3.0414 mL | 7.6034 mL | |
| 1 M HCl | 20 mM | 0.2281 mL | 1.1405 mL | 2.2810 mL | 5.7026 mL |
| 25 mM | 0.1825 mL | 0.9124 mL | 1.8248 mL | 4.5620 mL | |
| 30 mM | 0.1521 mL | 0.7603 mL | 1.5207 mL | 3.8017 mL | |
| 40 mM | 0.1141 mL | 0.5703 mL | 1.1405 mL | 2.8513 mL | |
| 50 mM | 0.0912 mL | 0.4562 mL | 0.9124 mL | 2.2810 mL | |
| 60 mM | 0.0760 mL | 0.3802 mL | 0.7603 mL | 1.9009 mL | |
| 80 mM | 0.0570 mL | 0.2851 mL | 0.5703 mL | 1.4256 mL | |
| 100 mM | 0.0456 mL | 0.2281 mL | 0.4562 mL | 1.1405 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
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