Art. -Nr.: HY-12115

NG-nitro-L-arginine

Name: NG-nitro-L-arginine
Brand: MedChemExpress
SKU: HY-12115
Price: 25.00 USD
Availability: InStock
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(Synonyms: Nω-nitro-L-arginine; L-NOARG; L-NG-Nitroarginine)

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NG-nitro-L-arginine (Nω-nitro-L-arginine; L-NOARG; L-NG-Nitroarginine) is a nitric oxide synthase (NO Synthase) inhibitor, with a K_d of 15 nM for constitutive NOS from bovine brain and a K_i of 4.4 μM for inducible NOS from mouse macrophages. NG-nitro-L-arginine competitively blocks the synthesis of NO from L-arginine; in addition, it inhibits endothelium-dependent vasodilation, and exhibits effects such as blood pressure elevation, heart rate reduction and vasoconstriction. NG-nitro-L-arginine regulates mitochondrial respiratory efficiency, antioxidant enzyme activity, lipid peroxidation level and tissue lead content in rats. NG-nitro-L-arginine can be used in studies related to cardiovascular diseases.

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Reinheit : 99.78%
CAS. Nr.: 2149-70-4
Formel: C₆H₁₃N₅O₄
Molecular Weight:219.20

Speicherung:

Store at room temperature 3 years.

In solvent -80°C, 2 years , -20°C, 1 year

NG-nitro-L-arginine Chemical Structure

CAS. Nr.: 2149-70-4

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Biologische Aktivität
- Beschreibung
- IC₅₀ & Target
- Cellular Effect
- In Vitro
- In Vivo
- Clinical Trial
Chemical Information
Lösungsmittel & Löslichkeit
- In Vitro
Reinheit & Dokumentation
Verweise
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

IC₅₀ & Target

^[3]

iNOS

4.4 μM (Ki)

Alle NO Synthase Isoform-spezifische Produkte anzeigen :

Cellular Effect

Cell Line	Type	Value	Description	References
HEK-293T	IC₅₀	5 μM Compound: L-NNA	Inhibition of rat nNOS expressed in HEK293T cells preincubated for 30 mins prior A23187-induced activation measured after 6 hrs by Griess assay Inhibition of rat nNOS expressed in HEK293T cells preincubated for 30 mins prior A23187-induced activation measured after 6 hrs by Griess assay	[PMID: 22370337]
RAW	IC₅₀	14 μM Compound: 1	Inhibition of radiolabeled arginine conversion to citrulline by isomeric form of Inducible nitric oxide synthase from mouse RAW 264.7 cells Inhibition of radiolabeled arginine conversion to citrulline by isomeric form of Inducible nitric oxide synthase from mouse RAW 264.7 cells	[PMID: 9651169]
RAW264.7	CC₅₀	>100 μM Compound: Nomega-nitro-L-arginine	Cytotoxicity against mouse RAW264.7 cells assessed as reduction in cell viability after 3 hrs by alamar blue assay Cytotoxicity against mouse RAW264.7 cells assessed as reduction in cell viability after 3 hrs by alamar blue assay	[PMID: 22148193]
RAW264.7	IC₅₀	143.55 μM Compound: Nomega-nitro-L-arginine	Inhibition of iNOS-mediated NO production in LPS-stimulated mouse RAW264.7 cells after 24 hrs by Griess reagent method Inhibition of iNOS-mediated NO production in LPS-stimulated mouse RAW264.7 cells after 24 hrs by Griess reagent method	[PMID: 22148193]
RAW264.7	IC₅₀	144.3 μM Compound: N-Omega-nitro-L-arginine	Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-stimulated nitric oxide production compound treated prior to LPS challenge by Griess assay Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-stimulated nitric oxide production compound treated prior to LPS challenge by Griess assay	[PMID: 27976895]
RAW264.7	IC₅₀	146.4 μM Compound: L-NNA	Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced nitric oxide production by spectrophotometric analysis Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced nitric oxide production by spectrophotometric analysis	[PMID: 24597849]
RAW264.7	IC₅₀	152.46 μM Compound: N-omega-nitro-L-arginine	Inhibition of iNOS in mouse RAW264.7 cells assessed as anti-inflammatory activity by measuring maximum inhibition of LPS-induced nitric oxide production after 24 hrs Inhibition of iNOS in mouse RAW264.7 cells assessed as anti-inflammatory activity by measuring maximum inhibition of LPS-induced nitric oxide production after 24 hrs	[PMID: 21846089]
RAW264.7	IC₅₀	2.918 μM Compound: L-NNA	Anti-inflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced nitric oxide synthase production measured after 24 hrs by ELISA Anti-inflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced nitric oxide synthase production measured after 24 hrs by ELISA	[PMID: 33065432]
RAW264.7	IC₅₀	37.7 μM Compound: L-NA	Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced nitric oxide production by measuring nitrite accumulation by Griess method Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced nitric oxide production by measuring nitrite accumulation by Griess method	[PMID: 28561586]
RAW264.7	IC₅₀	39.5 μM Compound: N-nitro-L-arginine	Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced NO production preincubated for 1 hrs followed by LPS stimulation and measured after 24 hrs by Griess reagent based spectrophotometrical method Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced NO production preincubated for 1 hrs followed by LPS stimulation and measured after 24 hrs by Griess reagent based spectrophotometrical method	[PMID: 33422907]
RAW264.7	IC₅₀	43.6 μM Compound: L-NA	Antiinflammatory activity against mouse RAW264.7 cells assessed as inhibition of LPS-induced nitric oxide production after 24 hrs by Griess reagent method Antiinflammatory activity against mouse RAW264.7 cells assessed as inhibition of LPS-induced nitric oxide production after 24 hrs by Griess reagent method	[PMID: 19715321]

In Vitro

NG-nitro-L-arginine (L-NG-Nitroarginine) acts as a rapidly reversible competitive inhibitor of iNOS in mouse macrophages, with a K_i value of 4.4 μM; it also functions as a slow-onset, high-affinity binding inhibitor of cNOS from bovine brain, with a K_d value of 15 nM, and L-arginine competes with NA for binding to the active site of cNOS^[3].
NG-nitro-L-arginine potently inhibits NADPH oxidation catalyzed by bovine brain cNOS, with an association rate constant of 3.2 × 10⁴ M^-1s^-1^[3].
NG-nitro-L-arginine dissociates completely from inhibited bovine brain cNOS upon reactivation with saturating concentrations of L-arginine^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Cardiovascular RegulationLocal Vascular RegulationMitochondrial Function

Cardiovascular Regulation

Local Vascular Regulation

NG-nitro-L-arginine (Nω-nitro-L-arginine) (0.05-2 mg per 100 g body weight per hour; intravenous injection; continuous infusion for 1 h) dose-dependently increases mean arterial pressure, with a median effective dose of 0.25 mg/100 gm body weight, and also reverses the blunted pressor response to vasopressors induced by pregnancy^[1].

Mitochondrial Function

NG-nitro-L-arginine (L-NNA) (35 mg/kg, i.p., 30 d pre/post lead) produces divergent hepatic effects in lead-poisoned rats based on hypoxia tolerance: it reduces liver lead only in low-tolerance rats post exposure, increases hepatic nitrate solely in high-tolerance rats, impairs NADH-dependent mitochondrial respiration in low-tolerance rats while restoring succinate-linked respiration in high-tolerance rats, exacerbates protein oxidation in low-tolerance rats, mitigates lipid peroxidation in high-tolerance rats, and elevates SOD/GR/GPx but suppresses CAT in high-tolerance rats^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Related Protocols :

Ferroptosis Solutions

Clinical Trial

NCT Number	Sponsor	Condition	Start Date	Phase A549
NCT01329991			2011-05	PHASE1

Chemical Information

CAS. Nr. 2149-70-4
Appearance Solid
Molecular Weight 219.20
Formel C₆H₁₃N₅O₄
Color White to off-white
SMILES

N[C@@H](CCCNC(N[N+]([O-])=O)=N)C(O)=O
Synonyms

Nω-nitro-L-arginine; L-NOARG; L-NG-Nitroarginine
Versand

Room temperature in continental US; may vary elsewhere.

Speicherung

Store at room temperature 3 years

In solvent	-80°C	2 years
	-20°C	1 year

Lösungsmittel & Löslichkeit

In Vitro:

1 M HCl : 100 mg/mL (456.20 mM; Need ultrasonic)

H₂O : 3.33 mg/mL (15.19 mM; ultrasonic and adjust pH to 2 with HCl)

DMSO : < 1 mg/mL (insoluble or slightly soluble)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

4.5620 mL

22.8102 mL

45.6204 mL

5 mM

0.9124 mL

4.5620 mL

9.1241 mL

10 mM

0.4562 mL

2.2810 mL

4.5620 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Working solution concentration: 0.22 mg/mL

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact

MedChemExpress (MCE) .

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (274 KB)
SDS (604 KB)
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COA (2659 KB)
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Handling Instructions (2659 KB)

Verweise

[1]. Molnár M, et al. Nω‑Nitro‑L‑arginine, an inhibitor of nitric oxide synthesis, increases blood pressure in rats and reverses the pregnancy‑induced refractoriness to vasopressor agents. American Journal of Obstetrics and Gynecology, 1992, 166(5): 1560‑1567.

[2]. Tkaczenko H, et al. Elucidation of the Role of L‑Arginine and Nω‑Nitro‑L‑Arginine in the Treatment of Rats with Different Levels of Hypoxic Tolerance and Exposure to Lead Nitrate. Cellular Physiology & Biochemistry, 2024.

[3]. Furfine ES, et al. Selective inhibition of constitutive nitric oxide synthase by L-NG-nitroarginine. Biochemistry. 1993 Aug 24;32(33):8512-7. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
H₂O / 1 M HCl	1 mM	4.5620 mL	22.8102 mL	45.6204 mL	114.0511 mL
	5 mM	0.9124 mL	4.5620 mL	9.1241 mL	22.8102 mL
	10 mM	0.4562 mL	2.2810 mL	4.5620 mL	11.4051 mL
	15 mM	0.3041 mL	1.5207 mL	3.0414 mL	7.6034 mL
1 M HCl	20 mM	0.2281 mL	1.1405 mL	2.2810 mL	5.7026 mL
	25 mM	0.1825 mL	0.9124 mL	1.8248 mL	4.5620 mL
	30 mM	0.1521 mL	0.7603 mL	1.5207 mL	3.8017 mL
	40 mM	0.1141 mL	0.5703 mL	1.1405 mL	2.8513 mL
	50 mM	0.0912 mL	0.4562 mL	0.9124 mL	2.2810 mL
	60 mM	0.0760 mL	0.3802 mL	0.7603 mL	1.9009 mL
	80 mM	0.0570 mL	0.2851 mL	0.5703 mL	1.4256 mL
	100 mM	0.0456 mL	0.2281 mL	0.4562 mL	1.1405 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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NG-nitro-L-arginine

Alle NO Synthase Isoform-spezifische Produkte anzeigen