Ninhydrin
Based on 1 Customer Validation
Ninhydrin can be used as a chromogenic analytical probe for the quantification of amino acids and proteins.
For research use only. We do not sell to patients.
- Purity: 99.99%
- CAS No.: 485-47-2
- Formula: C9H6O4
- Molecular Weight:178.14
-
Storage:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Chemical Information
-
Solvent & Solubility
- Purity & Documentation
- References
-
Help & FAQs
Biological Activity
IC50: chromogenic analytical probe[1]
Ninhydrin is used in amino acid analysis of proteins. Except proline, Most amino acids can be hydrolyzed and react with ninhydrin. The amino acids are then quantified colorimetrically after separation by chromatography. Ninhydrin reacts with primary and secondary amines producing a blue or purple reaction product: diketohydrindylidene-diketohydrindamine.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
-
CAS No. 485-47-2
-
Appearance Solid
-
Molecular Weight 178.14
-
Formula C9H6O4
-
Color White to light yellow
-
SMILES
O=C1C(O)(O)C(C2=C1C=CC=C2)=O
-
Shipping
Room temperature in continental US; may vary elsewhere.
-
Storage
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Solvent & Solubility
DMSO : 50 mg/mL (280.68 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : 25 mg/mL (140.34 mM; ultrasonic and warming and heat to 60°C)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
-
Data Sheet (278 KB)
-
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
-
Handling Instructions (2659 KB)
References
[1]. Omar MA, et al. Utility of ninhydrin reagent for spectrofluorimetric determination of heptaminol in human plasma.Luminescence. 2018 Sep;33(6):1107-1112. [Content Brief]
[2]. Anantharaman S, et al. Ninhydrin-sodium molybdate chromogenic analytical probe for the assay of amino acids and proteins.rSpectrochim Acta A Mol Biomol Spectrosc. 2017 Feb 15;173:897-903. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O / DMSO | 1 mM | 5.6136 mL | 28.0678 mL | 56.1356 mL | 140.3391 mL |
| 5 mM | 1.1227 mL | 5.6136 mL | 11.2271 mL | 28.0678 mL | |
| 10 mM | 0.5614 mL | 2.8068 mL | 5.6136 mL | 14.0339 mL | |
| 15 mM | 0.3742 mL | 1.8712 mL | 3.7424 mL | 9.3559 mL | |
| 20 mM | 0.2807 mL | 1.4034 mL | 2.8068 mL | 7.0170 mL | |
| 25 mM | 0.2245 mL | 1.1227 mL | 2.2454 mL | 5.6136 mL | |
| 30 mM | 0.1871 mL | 0.9356 mL | 1.8712 mL | 4.6780 mL | |
| 40 mM | 0.1403 mL | 0.7017 mL | 1.4034 mL | 3.5085 mL | |
| 50 mM | 0.1123 mL | 0.5614 mL | 1.1227 mL | 2.8068 mL | |
| 60 mM | 0.0936 mL | 0.4678 mL | 0.9356 mL | 2.3390 mL | |
| 80 mM | 0.0702 mL | 0.3508 mL | 0.7017 mL | 1.7542 mL | |
| 100 mM | 0.0561 mL | 0.2807 mL | 0.5614 mL | 1.4034 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Ninhydrin Related Classifications
HY-D0908 Related Classifications
Powered by Bioz