1. Dye Reagents
  2. Ninhydrin

Ninhydrin 

Cat. No.: HY-D0908
Handling Instructions

Ninhydrin can be used as a chromogenic analytical probe for the quantification of amino acids and proteins.

For research use only. We do not sell to patients.

Ninhydrin Chemical Structure

Ninhydrin Chemical Structure

CAS No. : 485-47-2

Size Price Stock Quantity
Free Sample (0.5-1 mg)   Apply Now  
Solution
10 mM * 1 mL in DMSO USD 55 In-stock
Estimated Time of Arrival: December 31
Solid + Solvent
10 mM * 1 mL
ready for reconstitution
USD 55 In-stock
Estimated Time of Arrival: December 31
Solid
500 mg USD 50 In-stock
Estimated Time of Arrival: December 31
1 g   Get quote  
5 g   Get quote  

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Customer Review

Based on 1 publication(s) in Google Scholar

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Description

Ninhydrin can be used as a chromogenic analytical probe for the quantification of amino acids and proteins.

IC50 & Target

IC50: chromogenic analytical probe[1]

In Vitro

Ninhydrin is used in amino acid analysis of proteins. Except proline, Most amino acids can be hydrolyzed and react with ninhydrin. The amino acids are then quantified colorimetrically after separation by chromatography.
Ninhydrin reacts with primary and secondary amines producing a blue or purple reaction product: diketohydrindylidene-diketohydrindamine.

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

178.14

Formula

C₉H₆O₄

CAS No.
Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

H2O : 25 mg/mL (140.34 mM; ultrasonic and warming and heat to 60°C)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 5.6136 mL 28.0678 mL 56.1356 mL
5 mM 1.1227 mL 5.6136 mL 11.2271 mL
10 mM 0.5614 mL 2.8068 mL 5.6136 mL
*Please refer to the solubility information to select the appropriate solvent.
References

Purity: ≥98.0%

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The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

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Product Name:
Ninhydrin
Cat. No.:
HY-D0908
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