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Cat. No.: HY-157926

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Nitroso-PSAP

Nitroso-PSAP is a sensitive and specific chromogenic reagent for iron (Fe²⁺), which is used for the colorimetric determination of iron in blood after mineralization with periodic acid. TNitroso-PSAP chelates with iron (Fe²⁺) to produce a green complex (λmax=756 nm, Epsilon = 4.5 x 10 4 dm 3 mol 1 cm 1 ).

For research use only. We do not sell to patients.

CAS No.: 80459-15-0
Formula: C₁₂H₁₈N₂O₅S
Molecular Weight:302.35

Storage:

Please store the product under the recommended conditions in the Certificate of Analysis.

Data Sheet (270 KB)

Nitroso-PSAP Chemical Structure

CAS No.: 80459-15-0

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Use of MCE Products

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity

Description

Chemical Information

CAS No. 80459-15-0
Molecular Weight 302.35
Formula C₁₂H₁₈N₂O₅S
SMILES

O=NC1=CC=C(N(CCCS(=O)(O)=O)CCC)C=C1O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Protocols

All Cell Biology

Mesenchymal stromal/stem cell osteogenic differentiation
Mesenchymal stromal/stem cells can be induced toward an osteoblast-like lineage in vitro by culture in osteogenic medium containing dexamethasone, ascorbic acid or ascorbate-2-phosphate, and β-glycerophosphate; the differentiation process is commonly evaluated by alkaline phosphatase activity, osteogenic marker expression, collagenous matrix formation, and calcium-rich matrix mineralization. The main readouts are alkaline phosphatase activity as an early osteogenic marker and Alizarin Red S staining as a calcium-deposit readout for mineralized extracellular matrix; Alizarin Red S can be inspected microscopically or extracted and measured colorimetrically at 405 nm.

Purity & Documentation

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Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (270 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. TAKAYANAGI M, et al. Direct Colorimetric Determination of Iron in Whole-Blood Based on Chelation with 2-Nitroso-5-(N-Propyl-N-Sulfopropylamino) Phenol (Nitroso-PSAP)[J]. Japanese Journal of Clinical Chemistry, 1985, 14(3): 155-160.

[2]. Yokota F, et al. Solid phase colorimetry of trace metal ions based on a tristimulus chromaticity diagram. Simultaneous determination of iron (II) and iron (III)[J]. Analytical Communications, 1997, 34(4): 111-112.

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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