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Cat. No.: HY-126774

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DAF-FM

1 Cited Publications

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Based on 1 publication(s) in Google Scholar

DAF-FM is a cell-impermeant diaminofluorescein, which can be used as fluorescent indicator for nitric oxide (NO) with good pH tolerance.

For research use only. We do not sell to patients.

Purity: 98.12%
CAS No.: 254109-20-1
Formula: C₂₁H₁₄F₂N₂O₅
Molecular Weight:412.34

Storage:

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

DAF-FM Chemical Structure

CAS No.: 254109-20-1

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity

Description

DAF-FM is a cell-impermeant diaminofluorescein, which can be used as fluorescent indicator for nitric oxide (NO) with good pH tolerance^[1].

Chemical Information

CAS No. 254109-20-1
Appearance Solid
Molecular Weight 412.34
Formula C₂₁H₁₄F₂N₂O₅
Color Light yellow to brown
SMILES

O=C1OC2(C3=C(OC4=C2C=C(F)C(O)=C4)C=C(O)C(F)=C3)C5=C1C(N)=C(NC)C=C5
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Publications (1)

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SSRN

2025 Aug 26.

Purity & Documentation

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Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (274 KB)
SDS (392 KB)
- English - EN (392 KB)
- Français - FR (392 KB)
- Deutsch - DE (392 KB)
- Norwegian - NO (392 KB)
- Español - ES (392 KB)
- Swedish - SV (392 KB)
- Italian - IT (392 KB)
- Korean - KR (392 KB)
- Portuguese - PT (392 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Kim WS, et al., Measuring nitric oxide in single neurons by capillary electrophoresis with laser-induced fluorescence: use of ascorbate oxidase in diaminofluorescein measurements. Anal Chem. 2006 Mar 15;78(6):1859-65. [Content Brief]

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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100 mg

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Biological Activity
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Chemical Information
Publications (1)
Purity & Documentation
References

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DAF-FM

Publications Citing Use of MedChemExpress (MCE) DAF-FM