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Cat. No.: HY-141415

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Tween 20

(Synonyms: Polysorbate 20)

7 Cited Publications

Customer Review

Based on 7 publication(s) in Google Scholar

Tween 20 (Polysorbate 20) is a polyoxyethylene (POE)-type nonionic surfactant.

For research use only. We do not sell to patients.

Purity: 98.0%
CAS No.: 9005-64-5

Storage:

Please store the product under the recommended conditions in the Certificate of Analysis.

Tween 20 Chemical Structure

CAS No.: 9005-64-5

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Top Publications Citing

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Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- In Vitro
Chemical Information
Publications (7)
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Calculators
Help & FAQs
Classifications
Bioz

Biological Activity

Description

Tween 20 (Polysorbate 20) is a polyoxyethylene (POE)-type nonionic surfactant^[1]^[2]^[3].

In Vitro

Addition of Tween 20 can modify lignin surface properties to change its hydrophobicity, hydrogen bonding ability and surface charges, thus reducing the non-productive adsorption of proteins^[1].
Tween 20 (0.35%-0.7% w/w) added to 0.05 mol/L of phosphate buffer pH 6.5 dramatically increased the apparent solubility of the drug from 0.8 μg/ml even to 353 μg/ml, while its effect in lower pH or in water was much smaller (20 μg/ml in pH 4.5). The increased concentration of phosphate salts (0.2 mol/l) at pH 6.5 in the presence of 0.7% of Tween 20, resulted in further increase of candesartan cilexetil solubility to 620 μg/ml^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS No. 9005-64-5
Appearance Viscous Liquid
Color Off-white to light yellow
SMILES

[Tween 20]
Synonyms

Polysorbate 20
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Publications (7)

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Cell

Stabilizing MARCH7 as a ferro-guardian against ferroptosis. [Abstract]

2026 Apr 27:S0092-8674(26)00388-0. PMID: 42049018
Autophagy

Byakangelicin alleviates metabolic dysfunction-associated steatohepatitis by selective inhibition of a non-canonical MTORC1 signaling pathway. [Abstract]

2026 May 25:1-21. PMID: 42152469
Adv Sci (Weinh)

A Novel tRF, HCETSR, Derived From tRNA-Glu/TTC, Inhibits HCC Malignancy by Regulating the SPBTN1-catenin Complex Axis. [Abstract]

2025 Feb 8:e2415229. PMID: 39921434
Neuropsychopharmacology

Small-molecule natural product sophoricoside reduces peripheral neuropathic pain via directly blocking of NaV1.6 in dorsal root ganglion nociceptive neurons. [Abstract]

2025 Mar;50(4):662-672. PMID: 39414988
Int J Pharm

A Nanoemulgel for Nose-to-Brain Delivery of Quetiapine - QbD-Enabled Formulation Development &In-vitro Characterization. [Abstract]

2023 Dec 15:648:123566. PMID: 37918496
Biochim Biophys Acta Mol Cell Res

MARCH5 ameliorates aortic valve calcification via RACGAP1-DRP1 associated mitochondrial quality control. [Abstract]

2025 Jan 27:119911. PMID: 39880131
Neuroscience

Tk2 deficiency-mediated mitochondrial dysfunction drives neuroinflammation and seizure frequency in epilepsy. [Abstract]

2026 Feb 16:595:171-186. PMID: 41500441

Solvent & Solubility

In Vitro:

DMSO : 50 mg/mL (ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 1.67 mg/mL; Clear solution

This protocol yields a clear solution of ≥ 1.67 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (16.7 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (271 KB)
SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Yu-An Chen, et al. Evaluation of the action of Tween 20 non-ionic surfactant during enzymatic hydrolysis of lignocellulose: Pretreatment, hydrolysis conditions and lignin structure. Bioresour Technol. 2018 Dec;269:329-338. [Content Brief]

[2]. Katarzyna Hoppe, et al. The effect of polysorbate 20 on solubility and stability of candesartan cilexetil in dissolution media. AAPS PharmSciTech. 2014 Oct;15(5):1116-25. [Content Brief]

[3]. JustynaŁuczak, et al. Micelle formation of Tween 20 nonionic surfactant in imidazolium ionic liquids. Colloids and Surfaces A: Physicochemical and Engineering Aspects. Volume 471, 20 April 2015, Pages 26-37.

Calculators

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Keywords

100 mg

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