1. Metabolic Enzyme/Protease
  2. E1/E2/E3 Enzyme
  3. NSC624206

NSC624206 

Cat. No.: HY-103436
Handling Instructions

NSC624206 is an inhibitor of ubiquitin E1 (UBA1), with an IC50 of ~9 μM. NSC624206 specifically blocks ubiquitin-thioester formation (IC50=13 μM) but has no effect on ubiquitin adenylation.

For research use only. We do not sell to patients.

NSC624206 Chemical Structure

NSC624206 Chemical Structure

CAS No. : 13116-77-3

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Description

NSC624206 is an inhibitor of ubiquitin E1 (UBA1), with an IC50 of ~9 μM. NSC624206 specifically blocks ubiquitin-thioester formation (IC50=13 μM) but has no effect on ubiquitin adenylation[1].

IC50 & Target

IC50: ~9 μM (ubiquitin E1)[1]

In Vitro

NSC624206 prevents in vitro ubiquitination of the cell cycle regulator p27 as well as inducing an accumulation of p27 in the HepG2 liver cancer cell line[1].
NSC624206 clearly reduces ubiquitin-E1 thioesters in a concentration-dependent manner with an IC50 of 13 μM[1].
NSC624206 (10 nM-10 μM; 24 hours) induces accumulation of p27 in cells[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis[1]

Cell Line: Kip16 cells
Concentration: 10 nM-10 μM
Incubation Time: 24 hours
Result: Induced accumulation of p27.
Molecular Weight

410.51

Formula

C₁₉H₃₃Cl₂NS₂

CAS No.

13116-77-3

SMILES

CCCCCCCCCCNCCSSCC1=CC=C(Cl)C=C1.[H]Cl

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Room temperature in continental US; may vary elsewhere.

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

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Keywords:

NSC624206NSC 624206NSC-624206E1/E2/E3 EnzymeE1 activating enzymeE2 conjugating enzymeE3 ligating enzymeUbiquitin activating enzymeUbiquitin conjugating enzymeUbiquitin ligaseubiquitinE1p27kip1proteolysisubiquitin-thioesterInhibitorinhibitorinhibit

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NSC624206
Cat. No.:
HY-103436
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