NSP-DMAE-NHS
Based on 1 Customer Validation
NSP-DMAE-NHS is an acridinium ester-based signal amplifier that can act as a luminescent probe to effectively improve the detection performance of biosensors. NSP-DMAE-NHS preserves the biochemical activity of biomolecules during the conjugation process. NSP-DMAE-NHS has been successfully applied to label mouse anti-human PI3 monoclonal antibodies for chemiluminescent immunoassays, as well as for pre-competitive chemiluminescent immunochromatographic detection of TP53 fusion proteins. NSP-DMAE-NHS can be used in detection studies of cancer and related biomarkers.
For research use only. We do not sell to patients.
- Purity: 99.48%
- CAS No.: 194357-64-7
- Formula: C30H26N2O9S
- Molecular Weight:590.60
-
Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
-
Biological Activity
-
Chemical Information
-
Solvent & Solubility
- Purity & Documentation
- References
-
Help & FAQs
Biological Activity
NSP-DMAE-NHS (1 mg; 1 h shaking, 15 min quenching) successfully conjugates to anti-TP53 antibody, forming a chemiluminescent immunoconjugate with a minimum detectable sensitivity of 1.8 × 10-17 mol[1].
NSP-DMAE-NHS-based competitive chemiluminescent reagent strip assay (30 μL conjugates; 30 min sample-conjugate incubation, 30 min strip incubation) enables ultrasensitive, selective detection of TP53 fusion protein with a limit of detection of 0.0008 nng/mL (0.013 pM), a linear range of 0.001 to 100 ng/mL, and an IC50 of ~0.24 ng/mL[1].
NSP-DMAE-NHS-labeled anti-human PI3 monoclonal antibody supports a highly reliable, sensitive, and specific CLIA assay for PI3 quantification[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
-
CAS No. 194357-64-7
-
Appearance Solid
-
Molecular Weight 590.60
-
Formula C30H26N2O9S
-
Color Light yellow to green yellow
-
SMILES
O=C(C1=CC(C)=C(OC(C2=C3C=CC=CC3=[N+](CCCS(=O)([O-])=O)C4=C2C=CC=C4)=O)C(C)=C1)ON5C(CCC5=O)=O
-
Shipping
Room temperature in continental US; may vary elsewhere.
-
Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Solvent & Solubility
DMSO : 10 mg/mL (16.93 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
-
Data Sheet (273 KB)
-
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Portuguese - PT (393 KB)
-
Handling Instructions (2659 KB)
References
[1]. Wang L, et al. Development of a Competitive Chemiluminescent Assay for Quantitative Determination of TP53 Fusion Protein Using Reagent Strips. Appl Biochem Biotechnol. 2024;196(9):6315-6329. [Content Brief]
[2]. Xu M, et al. Systematic analysis of serum peptidase inhibitor 3 in psoriasis diagnosis and treatment. Clin Rheumatol. 2024;43(11):3361-3372. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.6932 mL | 8.4660 mL | 16.9319 mL | 42.3298 mL |
| 5 mM | 0.3386 mL | 1.6932 mL | 3.3864 mL | 8.4660 mL | |
| 10 mM | 0.1693 mL | 0.8466 mL | 1.6932 mL | 4.2330 mL | |
| 15 mM | 0.1129 mL | 0.5644 mL | 1.1288 mL | 2.8220 mL |
Powered by Bioz