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  4. NSP-DMAE-NHS

NSP-DMAE-NHS is an acridinium ester-based signal amplifier that can act as a luminescent probe to effectively improve the detection performance of biosensors. NSP-DMAE-NHS preserves the biochemical activity of biomolecules during the conjugation process. NSP-DMAE-NHS has been successfully applied to label mouse anti-human PI3 monoclonal antibodies for chemiluminescent immunoassays, as well as for pre-competitive chemiluminescent immunochromatographic detection of TP53 fusion proteins. NSP-DMAE-NHS can be used in detection studies of cancer and related biomarkers.

For research use only. We do not sell to patients.

NSP-DMAE-NHS

NSP-DMAE-NHS Chemical Structure

CAS No. : 194357-64-7

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NSP-DMAE-NHS Related Antibodies

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  • Purity & Documentation

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Description

NSP-DMAE-NHS is an acridinium ester-based signal amplifier that can act as a luminescent probe to effectively improve the detection performance of biosensors. NSP-DMAE-NHS preserves the biochemical activity of biomolecules during the conjugation process. NSP-DMAE-NHS has been successfully applied to label mouse anti-human PI3 monoclonal antibodies for chemiluminescent immunoassays, as well as for pre-competitive chemiluminescent immunochromatographic detection of TP53 fusion proteins. NSP-DMAE-NHS can be used in detection studies of cancer and related biomarkers[1][2].

In Vitro

NSP-DMAE-NHS (1 mg; 1 h shaking, 15 min quenching) successfully conjugates to anti-TP53 antibody, forming a chemiluminescent immunoconjugate with a minimum detectable sensitivity of 1.8 × 10-17 mol[1].
NSP-DMAE-NHS-based competitive chemiluminescent reagent strip assay (30 μL conjugates; 30 min sample-conjugate incubation, 30 min strip incubation) enables ultrasensitive, selective detection of TP53 fusion protein with a limit of detection of 0.0008 nng/mL (0.013 pM), a linear range of 0.001 to 100 ng/mL, and an IC50 of ~0.24 ng/mL[1].
NSP-DMAE-NHS-labeled anti-human PI3 monoclonal antibody supports a highly reliable, sensitive, and specific CLIA assay for PI3 quantification[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

NSP-DMAE-NHS Related Antibodies
Formula

C30H26N2O9S
CAS No.
Appearance

Solid

Color

Light yellow to green yellow

SMILES

O=C(C1=CC(C)=C(OC(C2=C3C=CC=CC3=[N+](CCCS(=O)([O-])=O)C4=C2C=CC=C4)=O)C(C)=C1)ON5C(CCC5=O)=O
Molecular Weight

590.60

Shipping

Room temperature in continental US; may vary elsewhere.
Storage

-20°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility
In Vitro: 

DMSO : 10 mg/mL (16.93 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.6932 mL 8.4660 mL 16.9319 mL
5 mM 0.3386 mL 1.6932 mL 3.3864 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Purity & Documentation

Purity: 99.48%

SDS (393 KB)

COA (251 KB) HNMR (280 KB) RP-HPLC (252 KB) MS (69 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.6932 mL 8.4660 mL 16.9319 mL 42.3298 mL
5 mM 0.3386 mL 1.6932 mL 3.3864 mL 8.4660 mL
10 mM 0.1693 mL 0.8466 mL 1.6932 mL 4.2330 mL
15 mM 0.1129 mL 0.5644 mL 1.1288 mL 2.8220 mL
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NSP-DMAE-NHS Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

NSP-DMAE-NHS194357-64-7Biochemical Assay Reagentscancerchemiluminescence immunoassaysanti-TP53 antibodyimmunochromatographic detectionchemiluminescent assaysTP53 fusion proteinchemiluminescent immunoconjugateCLIA assayPI3mouse anti-human PI3 monoclonal antibodyInhibitorinhibitorinhibit

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