NSP-SA-NHS
Based on 1 Customer Validation
NSP-SA-NHS is an acridinium ester that can be used for chemiluminescent immunoassay. A rapid and sensitive chemiluminescent immunoassay of Bisphenol A (BPA) with NSP-SA-NHS-labeled has been developed.
For research use only. We do not sell to patients.
- Purity: 98.38%
- CAS No.: 199293-83-9
- Formula: C32H31N3O10S2
- Molecular Weight:681.73
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Storage:
-20°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Guidelines (The following is our recommended experimental protocol. This protocol is for reference only, and specific operations should be adjusted according to your actual needs).
NSP-SA-NHS labeling[1]
(1) Dissolve 1 mg BSA in 0.3 mL PBS (solution A), and dissolve 0.2 mg NSP-SA-NHS in 40 μL DMF (solution B).
(2) Add solution B to solution A, mix at 18°C and 130 rpm, and protect from light for 12 h.
(3) Dialyze against 500 mL distilled water at 4°C for 2 d, replacing the distilled water twice during this period (solution C).
(4) Slowly add solution B into solution C, mix at 18°C and 130 rpm protected from light for 24 h, and then dialyze against distilled water for 48 h. During this period, replace the distilled water four times.
(5) Dilute the conjugate to an appropriate concentration with distilled water and analyze using a UV spectrophotometer.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 199293-83-9
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Appearance Solid
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Molecular Weight 681.73
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Formula C32H31N3O10S2
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Color Light yellow to yellow
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SMILES
O=S(CCC[N+]1=C2C=CC=CC2=C(C(N(CCCC(ON3C(CCC3=O)=O)=O)S(=O)(C4=CC=C(C)C=C4)=O)=O)C5=C1C=CC=C5)([O-])=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
-20°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
Solvent & Solubility
DMSO : 4 mg/mL (5.87 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (276 KB)
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SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.4669 mL | 7.3343 mL | 14.6686 mL | 36.6714 mL |
| 5 mM | 0.2934 mL | 1.4669 mL | 2.9337 mL | 7.3343 mL |
NSP-SA-NHS Related Classifications
HY-D0893 Related Classifications
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