NucRFAP-2

NucRFAP-2 (2.5 μM) reveals dose-dependent FA sensing and HU-induced nuclear FA accumulation in FANCD2-deficient and WASp-deficient human T cells, with FANCD2-deficient cells reaching a ratio of 0.14^[1].

NucRFAP-2 binds the A/T-rich DNA hairpin structure with a K_D of 290 nM, supporting its application as a chromatin-anchoring nuclear probe^[1].
NucRFAP-2 (10 μM; 0-120 min) undergoes DNA-enhanced, FA-selective ratiometric response in aqueous buffer^[1].
NucRFAP-2 (2 μM; 1 h) localizes to the nucleus in live HEK-293 FlpIn cells, with Pearson coefficients of 0.74 and -0.54 for nuclear and cytosolic Halo-SNAP signals, respectively^[1].
NucRFAP-2 (2 μM; 0.5 h) reports a dose-dependent increase in nuclear FA in live HEK-293T cells exposed to 0-500 μM FA^[1].
NucRFAP-2 (2 μM; 0.5 h) detects elevated exogenous FA in the nuclei of wild-type MEF cells at 62.5 μM FA^[1].
NucRFAP-2 (2 μM; 1 h) detects elevated nuclear FA in ADH5-knockout MEF cells relative to wild-type MEF cells, consistent with the interplay between cytosolic and nuclear FA pools^[1].
NucRFAP-2 detects elevated baseline nuclear FA in WASp-deficient B cells and HU-induced nuclear FA accumulation in FANCD2-mutant human B cells^[1].