o,p'-DDE
o,p'-DDE (2,4-Dichlorodiphenyldichloroethylene) is a metabolite and degradation product of the organochlorine pesticide DDT. It accumulates in smallmouth buffalo, channel catfish, and largemouth bass, and in sediments from DDT manufacturing plants around the Huntsville Spring Branch-Indian Creek tributary system, where it is considered a persistent organic pollutant (POP). o,p'-DDE inhibits estrogen binding to the rainbow trout estrogen receptor (rtER) with an IC50 value of 3.2 μM. It induces concentration-dependent estradiol secretion in co-cultures of granulosa and theca cells isolated from porcine follicles. In ovo exposure to o,p'-DDE increases follicular degeneration and reduces testis size in Japanese medaka (O. latipes).
For research use only. We do not sell to patients.
- Purity: 99.95%
- CAS No.: 3424-82-6
- Formula: C14H8Cl4
- Molecular Weight:318.03
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Chemical Information
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CAS No. 3424-82-6
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Appearance Solid
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Molecular Weight 318.03
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Formula C14H8Cl4
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Color White to off-white
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SMILES
ClC1=CC=C(C=C1)/C(C2=CC=CC=C2Cl)=C(Cl)/Cl
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Synonyms
2,4'-DDE; 2,4-Dichlorodiphenyldichloroethylene; 2,4'-DDE; o,p'-Dichlorodiphenyldichloroethylene
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
DMSO : 100 mg/mL (314.44 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (274 KB)
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SDS (942 KB)
- English - EN (942 KB)
- Français - FR (942 KB)
- Deutsch - DE (942 KB)
- Norwegian - NO (942 KB)
- Español - ES (942 KB)
- Swedish - SV (942 KB)
- Italian - IT (942 KB)
- Portuguese - PT (942 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.1444 mL | 15.7218 mL | 31.4436 mL | 78.6089 mL |
| 5 mM | 0.6289 mL | 3.1444 mL | 6.2887 mL | 15.7218 mL | |
| 10 mM | 0.3144 mL | 1.5722 mL | 3.1444 mL | 7.8609 mL | |
| 15 mM | 0.2096 mL | 1.0481 mL | 2.0962 mL | 5.2406 mL | |
| 20 mM | 0.1572 mL | 0.7861 mL | 1.5722 mL | 3.9304 mL | |
| 25 mM | 0.1258 mL | 0.6289 mL | 1.2577 mL | 3.1444 mL | |
| 30 mM | 0.1048 mL | 0.5241 mL | 1.0481 mL | 2.6203 mL | |
| 40 mM | 0.0786 mL | 0.3930 mL | 0.7861 mL | 1.9652 mL | |
| 50 mM | 0.0629 mL | 0.3144 mL | 0.6289 mL | 1.5722 mL | |
| 60 mM | 0.0524 mL | 0.2620 mL | 0.5241 mL | 1.3101 mL | |
| 80 mM | 0.0393 mL | 0.1965 mL | 0.3930 mL | 0.9826 mL | |
| 100 mM | 0.0314 mL | 0.1572 mL | 0.3144 mL | 0.7861 mL |
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