PADI4_7 

PADI4_7 is an inert binder of peptidylarginine deiminase IV (PADI4) with a K_d value of 8 nM. PADI4_7 binds to PADI4 but does not modulate its catalytic activity. PADI4_7 can be functionalized into a biotinylated probe (bio-PADI4_7), which enriches intracellular PADI4 from cell extracts for biochemical analyses, including the identification of PADI4-interacting proteins under resting or activated conditions^[1][2].

PADI4_7 (30 μM) is an inert binder that does not modulate the catalytic activity of recombinant PADI4 in vitro^[1].
Biotinylated PADI4_7 (bio-PADI4_7) specifically enriches human PADI4 from lysates of mES cells stably expressing hPADI4, enabling the isolation of intracellular PADI4 for biochemical analysis^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

1707.95

C₈₄H₁₁₀N₁₈O₁₉S

{dTyr}-Tyr-Pro-Lys-Gly-Ser-Trp-Gly-Tyr-Lys-Leu-Phe-Cys-Gly-NH2 (thioether bond:dTyr1-Cys13)

{dTyr}-YPKGSWGYKLFCG-NH2 (thioether bond:dTyr1-Cys13)

O=C(N[C@@H](CC1=CC=C(C=C1)O)C(N2[C@@H](CCC2)C(N[C@@H](CCCCN)C(NCC(N[C@@H](CO)C(N[C@@H](CC3=CNC4=CC=CC=C34)C(NCC(N[C@@H](CC5=CC=C(C=C5)O)C(N[C@@H](CCCCN)C(N[C@@H](CC(C)C)C(N[C@@H](CC6=CC=CC=C6)C(N[C@@H](CSC7)C(NCC(N)=O)=O)=O)=O)=O)=O)=O)=O)=O)=O)=O)=O)=O)[C@@H](CC8=CC=C(C=C8)O)NC7=O

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Bertran MT, et al. A cyclic peptide toolkit reveals mechanistic principles of peptidylarginine deiminase IV regulation. Nat Commun. 2024;15(1):9746. Published 2024 Nov 11.

  [2]. Bertran M T, et al. A cyclic peptide toolkit reveals mechanistic principles of peptidylarginine deiminase IV (PADI4) regulation[J]. BioRxiv, 2023: 2023.12. 12.571217.