PE-Cy5.5
PE-Cy5.5 is a far-red emitting tandem dye designed based on the principle of fluorescence resonance energy transfer (FRET), and is widely used in multicolor flow cytometry. PE-Cy5.5 consists of phycoerythrin (PE) as the energy donor and the cyanine dye Cy5.5 as the energy acceptor. Upon excitation by blue or green laser light, PE absorbs energy and transfers it to Cy5.5 via FRET, ultimately resulting in Cy5.5 emitting characteristic far-red fluorescence (Ex/Em = 450-500 nm/698 nm).
For research use only. We do not sell to patients.
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
PE-Cy5.5 conjugates (0.25-5 μg/mL; 30 min at 4 °C) bind strongly and nonspecifically to mouse DEC205-positive CHO/mDEC205, KIT6/mDEC205, and HEK293/mDEC205 cells in proportion to surface mouse DEC205 expression, do not bind to human DEC205-positive cells, and exhibit stronger binding than other Cy5.5-based conjugates[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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SMILES
[PE-Cy5.5]
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
PE-Cy5.5 Related Classifications
HY-D3275 Related Classifications
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