Phenylalanylalanine
Based on 1 Customer Validation
Phenylalanylalanine (Phe-ala) is an enkephalinase inhibitor and belongs to the class of dipeptides. Phenylalanylalanine inhibits dipeptidyl carboxypeptidase-mediated enkephalin degradation by cleaving the Gly3-Phe4 bond, and when co-administered with Leu-enkephalin, it produces analgesic effects with a synergistic enhancement, which can be reversed by Naloxone (HY-17417A). Phenylalanylalanine can be used in pain-related research.
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- Pureté: 99.47%
- CAS No.: 3918-87-4
- Formule: C12H16N2O3
- Masse moléculaire:236.27
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Stockage:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Activité biologique
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Chemical Information
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Solvant et solubilité
- Pureté et documentation
- Références
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Help & FAQs
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HY-W009602R
Phenylalanylalanine (Standard) -
HY-P0224
N-Formyl-Met-Leu-Phe -
HY-15407
Sacubitril -
HY-W013375
Thiorphan -
HY-17620
Sacubitrilat -
HY-17399
Racecadotril -
HY-W015236
H-Gly-Gly-Gly-OH -
HY-W007970
H-Phe-Phe-OH -
HY-15407A
Sacubitril hemicalcium salt -
HY-19649
Candoxatril -
HY-10827
Kelatorphan -
HY-W142467
Tetraglycine
Activité biologique
Description
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| Caco-2 | IC50 |
0.18 μM
Compound: phe-ala
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Concentration required to inhibit the maximum [14C]Gly-Sar uptake by human Intestinal peptide transporter PepT1 in Caco-2 cells
Concentration required to inhibit the maximum [14C]Gly-Sar uptake by human Intestinal peptide transporter PepT1 in Caco-2 cells
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[PMID: 14761208] |
| MDCK | EC50 |
0.11 mM
Compound: 53
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Activation of human PEPT1 expressed in MDCK cells
Activation of human PEPT1 expressed in MDCK cells
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[PMID: 16759105] |
| MDCK | IC50 |
0.07 mM
Compound: 53
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Binding affinity to human PEPT1 assessed as inhibition of [14C]Gly-Sar uptake in MDCK cells
Binding affinity to human PEPT1 assessed as inhibition of [14C]Gly-Sar uptake in MDCK cells
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[PMID: 16759105] |
In Vitro
Phenylalanylalanine (Phe‑Ala) can undergo solid-state intramolecular cyclization to form cyclo (Phe‑Ala); cyclo (Phe‑Ala) can self-assemble into fibrous structures on the HOPG surface, exhibiting supramolecular gelation properties[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
In Vivo
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Swiss mice (Morini strain; female; 20-25 g)[1]
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Dosage:250 mg/kg (i.p. alone)
12.5 mg/kg (i.c.v. alone)
12.5 mg/kg (i.c.v. co-administered with 20 mcg Leu-enkephalin) -
Administration:i.p. (single dose)
i.c.v. (single dose) -
Result:Yielded an average response latency standard deviation of 3.1 sec across 13 observations after 250 mg/kg i.p. dosing, triggering analgesic responses exceeding the 45 sec threshold at 3 h and 5 h post‑administration.
Obtained an average response latency standard deviation of 4.3 sec across 14 observations after 12.5 mg/kg i.c.v. dosing, inducing analgesic responses exceeding the 45 sec threshold at 1.5 h, 2 h, 2.5 h and 10 h post‑administration.
Achieved an average response latency standard deviation of 4.6 sec across 14 observations after 12.5 mg/kg i.c.v. co‑administration with 20 mcg Leu‑enkephalin, generating prolonged and enhanced analgesic responses exceeding the 45 sec threshold from 1.5 h to 10 h post‑administration compared with separate i.c.v. injection.
Displayed analgesic effects reversible by naloxone, with latency recovering to baseline within 5-10 min.
Chemical Information
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CAS No. 3918-87-4
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Appearance Solid
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Masse moléculaire 236.27
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Formule C12H16N2O3
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Color White to off-white
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SMILES
C[C@@H](C(O)=O)NC([C@H](CC1=CC=CC=C1)N)=O
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Synonyms
Phe-ala
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Structure Classification
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Initial Source
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Solvant et solubilité
In Vitro:
H2O : 100 mg/mL (423.24 mM; Need ultrasonic)
DMSO : 4.35 mg/mL (18.41 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Pureté et documentation
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Fiche technique (272 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Instruction de manipulation (2659 KB)
Références
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO / H2O | 1 mM | 4.2324 mL | 21.1622 mL | 42.3245 mL | 105.8111 mL |
| 5 mM | 0.8465 mL | 4.2324 mL | 8.4649 mL | 21.1622 mL | |
| 10 mM | 0.4232 mL | 2.1162 mL | 4.2324 mL | 10.5811 mL | |
| 15 mM | 0.2822 mL | 1.4108 mL | 2.8216 mL | 7.0541 mL | |
| H2O | 20 mM | 0.2116 mL | 1.0581 mL | 2.1162 mL | 5.2906 mL |
| 25 mM | 0.1693 mL | 0.8465 mL | 1.6930 mL | 4.2324 mL | |
| 30 mM | 0.1411 mL | 0.7054 mL | 1.4108 mL | 3.5270 mL | |
| 40 mM | 0.1058 mL | 0.5291 mL | 1.0581 mL | 2.6453 mL | |
| 50 mM | 0.0846 mL | 0.4232 mL | 0.8465 mL | 2.1162 mL | |
| 60 mM | 0.0705 mL | 0.3527 mL | 0.7054 mL | 1.7635 mL | |
| 80 mM | 0.0529 mL | 0.2645 mL | 0.5291 mL | 1.3226 mL | |
| 100 mM | 0.0423 mL | 0.2116 mL | 0.4232 mL | 1.0581 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Phenylalanylalanine Related Classifications
HY-W009602 Related Classifications
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