Photoclick cholesterol 

Photoclick cholesterol is a photoreactive cholesterol analog capable of undergoing click reactions. Photoclick cholesterol enables specific, saturable photoaffinity labeling of the mitochondrial translocator protein TSPO^[1].

Photoclick cholesterol (10-25 μM; 30 min dark incubation, 10 min UV irradiation) binds directly and competitively to native 18 kDa TSPO in mitochondria-enriched fractions from MA-10 mouse tumour Leydig cells, with increased labelling at 25 μM compared to 10 μM, and reduced labelling in the presence of 20- or 65-fold molar excess cholesterol^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

482.70

C₃₀H₄₆N₂O₃

1485490-47-8

Liquid

Colorless to light yellow

C[C@]12[C@@](C[C@H](CC2)O)([H])C3(N=N3)C[C@]4([H])[C@]1([H])CC[C@]5([C@@]4([H])CC[C@]5([H])[C@H](C)CCC(OCCCCC#C)=O)C

Room temperature in continental US; may vary elsewhere.

Solution, -20°C, 2 years

• [1]. Georges E, et al. Direct and specific binding of cholesterol to the mitochondrial translocator protein (TSPO) using PhotoClick cholesterol analogue. J Biochem. 2021;170(2):239-243.  [Content Brief]