2. Metabolic Enzyme/Protease
  3. Glycosidase
  4. PNGase A

PNGase A is a β-aspartylglucosaminidase and N-glycan-releasing enzyme. PNGase A catalyzes hydrolysis reactions to release the N-glycan moiety from glycoproteins or glycopeptides. PNGase A releases N-linked oligosaccharides containing core α-1,3 fucose from glycopeptides. PNGase A undergoes self-deglycosylation, which may cause contamination of endogenous glycan structures in N-glycan analysis. PNGase A cannot be heterologously expressed in recombinant expression systems; it can be extracted and purified from almond seeds.

For research use only. We do not sell to patients.

PNGase A

PNGase A Chemical Structure

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150 U In-stock

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Based on 1 publication(s) in Google Scholar

PNGase A Related Antibodies

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Description

PNGase A is a β-aspartylglucosaminidase and N-glycan-releasing enzyme. PNGase A catalyzes hydrolysis reactions to release the N-glycan moiety from glycoproteins or glycopeptides. PNGase A releases N-linked oligosaccharides containing core α-1,3 fucose from glycopeptides. PNGase A undergoes self-deglycosylation, which may cause contamination of endogenous glycan structures in N-glycan analysis. PNGase A cannot be heterologously expressed in recombinant expression systems; it can be extracted and purified from almond seeds[1].

In Vitro

PNGase A (0.5 mU; overnight) releases core α1,3-fucosylated N-glycans from HRP glycoprotein and glycopeptides, but has lower efficiency for non-core α1,3-fucosylated HRP N-glycans and introduces contaminating N-glycans via self-deglycosylation[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

PNGase A Related Antibodies
Appearance

Liquid

Color

Colorless to light yellow

SMILES

[PNGase A]
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
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PNGase A Related Classifications

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This equation is commonly abbreviated as: C1V1 = C2V2

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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

PNGase AGlycosidasealmond seedsglycoproteinsN-glycanself-deglycosylationcore α-13 fucosecore α13-fucosylated N-glycansHRP glycoproteinβ-aspartylglucosaminyl bond hydrolaseglycopeptidesInhibitorinhibitorinhibit

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