pNP-ADPr disodium
Based on 1 Customer Validation
pNP-ADPr disodium is a colorimetric substrate that used for the first continuous Poly(ADP-ribose) glycohydrolase (PARG) and ADP-ribosyl hydrolase 3 (ARH3) activity assays. pNP-ADPr disodium can be used for the research of poly(ADP-ribose)polymerase (PARP) enzymes.
For research use only. We do not sell to patients.
- Purity: 98.08%
- Formula: C21H24N6Na2O16P2
- Molecular Weight:724.37
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Storage:
-20°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
pNP-ADPr is a colorimetric substrate that used for the first continuous Poly(ADP-ribose) glycohydrolase (PARG) and ADP-ribosyl hydrolase 3 (ARH3) activity assays[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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Appearance Solid
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Molecular Weight 724.37
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Formula C21H24N6Na2O16P2
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Color White to off-white
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SMILES
O[C@H]1[C@@H](O)[C@H](N2C3=NC=NC(N)=C3N=C2)O[C@@H]1COP(OP(OC[C@@H]4[C@@H](O)[C@@H](O)[C@H](OC5=CC=C([N+]([O-])=O)C=C5)O4)(O[Na])=O)(O[Na])=O
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Synonyms
ADP-ribose-pNP disodium
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
-20°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
Solvent & Solubility
H2O : 250 mg/mL (345.13 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (271 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
[1]. Drown BS, et al. Monitoring Poly(ADP-ribosyl)glycohydrolase Activity with a Continuous Fluorescent Substrate. Cell Chem Biol. 2018;25(12):1562-1570.e19. [Content Brief]
[2]. Nottbohm AC, et al. A colorimetric substrate for poly(ADP-ribose) polymerase-1, VPARP, and tankyrase-1. Angew Chem Int Ed Engl. 2007;46(12):2066-2069. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O | 1 mM | 1.3805 mL | 6.9025 mL | 13.8051 mL | 34.5127 mL |
| 5 mM | 0.2761 mL | 1.3805 mL | 2.7610 mL | 6.9025 mL | |
| 10 mM | 0.1381 mL | 0.6903 mL | 1.3805 mL | 3.4513 mL | |
| 15 mM | 0.0920 mL | 0.4602 mL | 0.9203 mL | 2.3008 mL | |
| 20 mM | 0.0690 mL | 0.3451 mL | 0.6903 mL | 1.7256 mL | |
| 25 mM | 0.0552 mL | 0.2761 mL | 0.5522 mL | 1.3805 mL | |
| 30 mM | 0.0460 mL | 0.2301 mL | 0.4602 mL | 1.1504 mL | |
| 40 mM | 0.0345 mL | 0.1726 mL | 0.3451 mL | 0.8628 mL | |
| 50 mM | 0.0276 mL | 0.1381 mL | 0.2761 mL | 0.6903 mL | |
| 60 mM | 0.0230 mL | 0.1150 mL | 0.2301 mL | 0.5752 mL | |
| 80 mM | 0.0173 mL | 0.0863 mL | 0.1726 mL | 0.4314 mL | |
| 100 mM | 0.0138 mL | 0.0690 mL | 0.1381 mL | 0.3451 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
pNP-ADPr disodium Related Classifications
HY-134354A Related Classifications
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