Pregnanolone sulfate

Pregnanolone sulfate (100 μM; 2 min) enhances spontaneous glutamate release in isolated excitatory hippocampal neurons, increasing mEPSC frequency by ~3-fold^[1].
Pregnanolone sulfate (100 μM; 2 min) inhibits postsynaptic NMDA receptor-mediated currents but has no net effect on NMDA receptor-mediated eEPSCs, suggesting that presynaptic enhancement of glutamate release compensates for postsynaptic NMDA receptor inhibition^[1].
Pregnanolone sulfate (0.1 mM) selectively prolongs the three longest closed-time components (τ_EC3-5) of GluN1/GluN2A receptor closures by 100-200%, without affecting open-interval distributions or modal gating kinetics^[3].
Pregnanolone sulfate (100 μM; 40 min) does not translocate GFP-tagged Munc13-1 to the plasma membrane of HEK293 cells^[1].
Pregnanolone sulfate (30-300 μM) inhibits macroscopic NMDA receptor currents in HEK 293 cells expressing GluN1/GluN2A receptors in a concentration-dependent and reversible manner, with an IC₅₀ of 67 μM^[3].
Pregnanolone sulfate (0.1 mM) increases macroscopic desensitization of GluN1/GluN2A receptors in HEK 293 cells, decreasing the I_ss/I_pk ratio from 0.48 to 0.08^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. .