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  4. Probe-Cys

Probe-Cys is a water-soluble and selective near-infrared fluorescent probe for Cysteine (Cys) (λex= 680 nm, λem=710 nm) that is not interfered by Hcy, GSH, and HS-. Probe-Cys can react with the stimulant DTT (HY-15917) and the NEM (HY-D0843) in HepG2 cells and zebrafish for the detection of endogenous Cys. Probe-Cys can also be used for imaging Cys in Arabidopsis thaliana. Probe-Cys provides a method for cancer diagnosis and exploration of plant sulfur metabolism.

For research use only. We do not sell to patients.

Probe-Cys

Probe-Cys Chemical Structure

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Probe-Cys Related Antibodies

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Description

Probe-Cys is a water-soluble and selective near-infrared fluorescent probe for Cysteine (Cys) (λex= 680 nm, λem=710 nm) that is not interfered by Hcy, GSH, and HS-. Probe-Cys can react with the stimulant DTT (HY-15917) and the NEM (HY-D0843) in HepG2 cells and zebrafish for the detection of endogenous Cys. Probe-Cys can also be used for imaging Cys in Arabidopsis thaliana. Probe-Cys provides a method for cancer diagnosis and exploration of plant sulfur metabolism[1].

In Vitro

Probe-Cys sensitively detects Cys in cell-free systems with a linear range of 0-100 μM, a detection limit of 0.035 μM, and a strong fluorescence turn-on response at 710 nm[1].
Probe-Cys (10 μM; 1 h) specifically detects endogenous and exogenous Cys in HepG2 cells, with no interference from Hcy or GSH, and responds to modulations in cellular Cys levels via DTT and NEM treatment[1].
Probe-Cys (10 μM; 1 h) penetrates Arabidopsis thaliana root tissue to detect endogenous Cys, with fluorescence responding to DTT-mediated elevation and NEM-mediated inhibition, and reveals higher Cys content in root meristematic zones compared to elongation zones[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Probe-Cys Related Antibodies
In Vivo

Probe-Cys (20 μM; 1 h) exhibits good permeability and detects endogenous and exogenous Cys in healthy zebrafish, with DTT (HY-15917) -pretreated zebrafish showing 96.61% of the fluorescence intensity of Cysteine (HY-Y0337)-pretreated zebrafish[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Zebrafish (3 days old)[1]
Dosage: 20 μM
Administration: incubation; 1 h
Result: Exhibited weak fluorescence with relative pixel intensity ca.
32.62% (defining the Cys-pretreated group intensity as 1.0).
Showed greatly reduced fluorescence with relative pixel intensity ca. 7.54% when zebrafish were preincubated with NEM (HY-D0843).
Showed obvious fluorescence enhancement with relative pixel intensity ca. 96.61% when zebrafish were preincubated with DTT.
Produced stronger fluorescence signals in the yolk sac compared to other areas.
Molecular Weight

585.67

Formula

C33H31NO7S
Appearance

Solid

Color

Brown to reddish brown

SMILES

O=C(C1=CC=CO1)OC2=CC(OC3=C4/C=C/C(C(C)(C5=CC=CC=C56)C)=[N+]6CCCCS(=O)([O-])=O)=C(C=C3CC4)C=C2
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

-20°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 50 mg/mL (85.37 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.7074 mL 8.5372 mL 17.0745 mL
5 mM 0.3415 mL 1.7074 mL 3.4149 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.7074 mL 8.5372 mL 17.0745 mL 42.6862 mL
5 mM 0.3415 mL 1.7074 mL 3.4149 mL 8.5372 mL
10 mM 0.1707 mL 0.8537 mL 1.7074 mL 4.2686 mL
15 mM 0.1138 mL 0.5691 mL 1.1383 mL 2.8457 mL
20 mM 0.0854 mL 0.4269 mL 0.8537 mL 2.1343 mL
25 mM 0.0683 mL 0.3415 mL 0.6830 mL 1.7074 mL
30 mM 0.0569 mL 0.2846 mL 0.5691 mL 1.4229 mL
40 mM 0.0427 mL 0.2134 mL 0.4269 mL 1.0672 mL
50 mM 0.0341 mL 0.1707 mL 0.3415 mL 0.8537 mL
60 mM 0.0285 mL 0.1423 mL 0.2846 mL 0.7114 mL
80 mM 0.0213 mL 0.1067 mL 0.2134 mL 0.5336 mL
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Probe-Cys Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Probe-CysFluorescent DyeN-ethylmaleimideArabidopsis thaliana1,4-dimercaptothreitolglutathionecancer cellszebrafishhomocysteineHepG2 cellsmeristematic zonescysteineInhibitorinhibitorinhibit

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