Probe-Cys
Based on 1 Customer Validation
Probe-Cys is a water-soluble and selective near-infrared fluorescent probe for Cysteine (Cys) (λex= 680 nm, λem=710 nm) that is not interfered by Hcy, GSH, and HS-. Probe-Cys can react with the stimulant DTT (HY-15917) and the NEM (HY-D0843) in HepG2 cells and zebrafish for the detection of endogenous Cys. Probe-Cys can also be used for imaging Cys in Arabidopsis thaliana. Probe-Cys provides a method for cancer diagnosis and exploration of plant sulfur metabolism.
For research use only. We do not sell to patients.
- Purity: 98.87%
- Formula: C33H31NO7S
- Molecular Weight:585.67
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Storage:
-20°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Probe-Cys sensitively detects Cys in cell-free systems with a linear range of 0-100 μM, a detection limit of 0.035 μM, and a strong fluorescence turn-on response at 710 nm[1].
Probe-Cys (10 μM; 1 h) specifically detects endogenous and exogenous Cys in HepG2 cells, with no interference from Hcy or GSH, and responds to modulations in cellular Cys levels via DTT and NEM treatment[1].
Probe-Cys (10 μM; 1 h) penetrates Arabidopsis thaliana root tissue to detect endogenous Cys, with fluorescence responding to DTT-mediated elevation and NEM-mediated inhibition, and reveals higher Cys content in root meristematic zones compared to elongation zones[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Zebrafish (3 days old)[1]
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Dosage:20 μM
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Administration:incubation; 1 h
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Result:Exhibited weak fluorescence with relative pixel intensity ca.
32.62% (defining the Cys-pretreated group intensity as 1.0).
Showed greatly reduced fluorescence with relative pixel intensity ca. 7.54% when zebrafish were preincubated with NEM (HY-D0843).
Showed obvious fluorescence enhancement with relative pixel intensity ca. 96.61% when zebrafish were preincubated with DTT.
Produced stronger fluorescence signals in the yolk sac compared to other areas.
Chemical Information
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Appearance Solid
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Molecular Weight 585.67
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Formula C33H31NO7S
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Color Brown to reddish brown
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SMILES
O=C(C1=CC=CO1)OC2=CC(OC3=C4/C=C/C(C(C)(C5=CC=CC=C56)C)=[N+]6CCCCS(=O)([O-])=O)=C(C=C3CC4)C=C2
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
-20°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Solvent & Solubility
DMSO : 50 mg/mL (85.37 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (277 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.7074 mL | 8.5372 mL | 17.0745 mL | 42.6862 mL |
| 5 mM | 0.3415 mL | 1.7074 mL | 3.4149 mL | 8.5372 mL | |
| 10 mM | 0.1707 mL | 0.8537 mL | 1.7074 mL | 4.2686 mL | |
| 15 mM | 0.1138 mL | 0.5691 mL | 1.1383 mL | 2.8457 mL | |
| 20 mM | 0.0854 mL | 0.4269 mL | 0.8537 mL | 2.1343 mL | |
| 25 mM | 0.0683 mL | 0.3415 mL | 0.6830 mL | 1.7074 mL | |
| 30 mM | 0.0569 mL | 0.2846 mL | 0.5691 mL | 1.4229 mL | |
| 40 mM | 0.0427 mL | 0.2134 mL | 0.4269 mL | 1.0672 mL | |
| 50 mM | 0.0341 mL | 0.1707 mL | 0.3415 mL | 0.8537 mL | |
| 60 mM | 0.0285 mL | 0.1423 mL | 0.2846 mL | 0.7114 mL | |
| 80 mM | 0.0213 mL | 0.1067 mL | 0.2134 mL | 0.5336 mL |
Probe-Cys Related Classifications
HY-D2315 Related Classifications
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