Propargylcholine bromide

Name: Propargylcholine bromide
Brand: MedChemExpress
SKU: HY-129084
Rating: 4.5 (1 reviews)

Cat. No.: HY-129084 Purity: 98.85%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Propargylcholine bromide is a choline analogue containing terminal propargyl that can be incorporated into all classes of Choline-containing phospholipids such as phosphatidylcholine and sphingomyelin, labeling Choline-containing phospholipids. Propargylcholine bromide-labeled phospholipid molecules can be visualized in cells with high sensitivity and spatial resolution. Propargylcholine bromide can be used as a molecular tool to study the biochemical and metabolic processes of Choline-containing phospholipids in cells. Propargylcholine (bromide) is a click chemistry reagent, it contains an Alkyne group and can undergo copper-catalyzed azide-alkyne cycloaddition (CuAAc) with molecules containing Azide groups.

For research use only. We do not sell to patients.

Propargylcholine bromide Chemical Structure

CAS No. : 111755-76-1

Size	Price	Stock	Quantity
5 mg	USD 32	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 50	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 110	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 180	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 340	In-stock	Estimated Time of Arrival: December 31
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Based on 1 publication(s) in Google Scholar

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Biological Activity
Purity & Documentation
References
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Description

Propargylcholine bromide is a choline analogue containing terminal propargyl that can be incorporated into all classes of Choline-containing phospholipids such as phosphatidylcholine and sphingomyelin, labeling Choline-containing phospholipids. Propargylcholine bromide-labeled phospholipid molecules can be visualized in cells with high sensitivity and spatial resolution. Propargylcholine bromide can be used as a molecular tool to study the biochemical and metabolic processes of Choline-containing phospholipids in cells^[1]^[2]. Propargylcholine (bromide) is a click chemistry reagent, it contains an Alkyne group and can undergo copper-catalyzed azide-alkyne cycloaddition (CuAAc) with molecules containing Azide groups.

In Vitro

Propargylcholine bromide (10, 50 µM; 24 h) incorporates into NIH 3T3 cells and shows strong staining in a concentration-dependent manner (fixed and stained with Alexa568-azide)^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Immunofluorescence^[1]

Cell Line:	NIH 3T3 cells
Concentration:	10, 50 µM
Incubation Time:	24 h
Result:	Showed strong staining proportional in intensity to the concentration of added Propargylcholine bromide.

In Vivo

Propargylcholine bromide (P-Cho; 3.5-4.0 mg/kg; i.p; single daily for 6 days) effectively incorporates into newly synthesized myelin within 1 week of dosing and sustained presence beyond 6 weeks in rhesus monkey^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

208.10

Formula

C₇H₁₄BrNO

CAS No.

111755-76-1

Appearance

Solid

Color

Off-white to yellow

SMILES

C#CC[N+](C)(CCO)C.[Br-]

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

DMSO : 125 mg/mL (600.67 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 125 mg/mL (600.67 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	4.8054 mL	24.0269 mL	48.0538 mL
5 mM	0.9611 mL	4.8054 mL	9.6108 mL
10 mM	0.4805 mL	2.4027 mL	4.8054 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 98.85%

Select Batch:

Data Sheet (271 KB) SDS (251 KB)

COA (245 KB) HNMR (216 KB) RP-HPLC (223 KB) MS (67 KB)

Handling Instructions (2659 KB)

References

[1]. Jao CY, et al. Metabolic labeling and direct imaging of choline phospholipids in vivo. Proc Natl Acad Sci U S A. 2009 Sep 8;106(36):15332-7. [Content Brief]

[2]. Karen R. Bottenfield, et al. Optimization of Propargylcholine to Label Newly Synthesized Myelin in the Rhesus Monkey Brain. FASEB J. 2020, 34(S1): 1-1.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO / H₂O	1 mM	4.8054 mL	24.0269 mL	48.0538 mL	120.1345 mL
	5 mM	0.9611 mL	4.8054 mL	9.6108 mL	24.0269 mL
	10 mM	0.4805 mL	2.4027 mL	4.8054 mL	12.0135 mL
	15 mM	0.3204 mL	1.6018 mL	3.2036 mL	8.0090 mL
	20 mM	0.2403 mL	1.2013 mL	2.4027 mL	6.0067 mL
	25 mM	0.1922 mL	0.9611 mL	1.9222 mL	4.8054 mL
	30 mM	0.1602 mL	0.8009 mL	1.6018 mL	4.0045 mL
	40 mM	0.1201 mL	0.6007 mL	1.2013 mL	3.0034 mL
	50 mM	0.0961 mL	0.4805 mL	0.9611 mL	2.4027 mL
	60 mM	0.0801 mL	0.4004 mL	0.8009 mL	2.0022 mL
	80 mM	0.0601 mL	0.3003 mL	0.6007 mL	1.5017 mL
	100 mM	0.0481 mL	0.2403 mL	0.4805 mL	1.2013 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Propargylcholine111755-76-1OthersNIH 3T3 cellsAlexa568propargylphosphatidylcholinesphingomyelinphospholipidsInhibitorinhibitorinhibit

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Propargylcholine bromide

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