Investigation of substance P transport across the blood-brain barrier

  • Peptides. 2002 Jan;23(1):157-65. doi: 10.1016/s0196-9781(01)00592-7.
Anita L Freed  1 ,  Kenneth L Audus ,  Susan M Lunte
Affiliations
  • 1. Department of Pharmaceutical Chemistry, The University of Kansas, School of Pharmacy, Lawrence KS 66047, USA. [email protected]
PMID: 11814631 DOI: 10.1016/s0196-9781(01)00592-7
Abstract

The transport of substance P (SP) was investigated using the bovine brain microvessel endothelial Cell Culture model of the blood-brain barrier (BBB). The samples were derivatized precolumn with naphthalene dialdehyde, then analyzed by cyclodextrin-modified micellar electrokinetic chromatography with laser-induced fluorescence detection. SP crossed the BBB in both the apical-to-basolateral and basolateral-to-apical directions through an active transport mechanism. The transport of SP from the apical side was demonstrated to be via transcytosis. The N-terminal (SP(1-4)) and C-terminal (SP(3-11)) fragments were also found to permeate the BBB from the apical side.

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