Isolation of xanthyletin, an inhibitor of ants' symbiotic fungus, by high-speed counter-current chromatography

  • J Chromatogr A. 2009 May 8;1216(19):4307-12. doi: 10.1016/j.chroma.2009.02.066.
Cristiane de Melo Cazal  1 ,  Vanessa de Cássia Domingues ,  Jaqueline Raquel Batalhão ,  Odair Corrêa Bueno ,  Edson Rodrigues Filho ,  Maria Fátima G Fernandes da Silva ,  Paulo Cezar Vieira ,  João Batista Fernandes
Affiliations
  • 1. Universidade Federal de São Carlos, Departamento de Química, SP, Brazil.
PMID: 19296958 DOI: 10.1016/j.chroma.2009.02.066
Abstract

Xanthyletin, an inhibitor of symbiotic fungus (Leucoagaricus gongylophorus) of leaf-cutting ant (Atta sexdens rubropilosa), as well as suberosin, seselin and xanthoxyletin were isolated from Citrus sinensis grafted on Citrus limonia. A two-phase solvent system composed of hexane/ethanol/acetonitrile/water (10:8:1:1, v/v) was used for the high-speed counter-current chromatographic isolation of xanthyletin with high yield and over 99% purity as determined by liquid and gas chromatography with mass spectrometry detection. Identifications were performed by UV spectra, IR spectra, (1)H NMR and (13)C NMR.

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