Determination of pinostrobin in rat plasma by LC-MS/MS: application to pharmacokinetics

  • J Pharm Biomed Anal. 2011 Dec 5;56(4):841-5. doi: 10.1016/j.jpba.2011.07.038.
Xin Hua  1 ,  Yu-Jie Fu ,  Yuan-Gang Zu ,  Lin Zhang ,  Wei Wang ,  Meng Luo
Affiliations
  • 1. Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, Harbin 150040, PR China.
PMID: 21840667 DOI: 10.1016/j.jpba.2011.07.038
Abstract

A rapid and sensitive method for the determination of pinostrobin in rat plasma was developed using liquid chromatography tandem mass spectrometry (LC-MS/MS) for the first time. Isoliquiritigenin was used as an internal standard in rat plasma. Chromatographic separation was performed on an HiQ Sil C(18) column with isocratic elution at a flow rate of 1mL/min. The mobile phase consisted of water and methanol (9:91, v/v) containing 0.1% formic acid. The quantification limit was 10ng/mL within a linear range of 10-1000ng/mL (R=0.9984). The intra- and inter-day assay precision ranged from 3.8-5.3% to 3.2-5.2%, respectively, and the intra- and inter-day assay accuracy was between 93.2-95.1% and 95.5-104.3%, respectively. Our results indicated that the LC-MS/MS method is effective for pharmacokinetic study of pinostrobin in rat plasma.

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