Isolation and structural elucidation of cyclic tetrapeptides from Onychocola sclerotica

  • J Nat Prod. 2012 Jun 22;75(6):1210-4. doi: 10.1021/np3000987.
Ignacio Pérez-Victoria  1 ,  Jesús Martín ,  Víctor González-Menéndez ,  Nuria de Pedro ,  Noureddine El Aouad ,  Francisco Javier Ortiz-López ,  José Rubén Tormo ,  Gonzalo Platas ,  Francisca Vicente ,  Gerald F Bills ,  Olga Genilloud ,  Michael A Goetz ,  Fernando Reyes
Affiliations
  • 1. Fundación MEDINA, Centro de Excelencia en Investigación de Medicamentos Innovadores en Andalucía, Avenida del Conocimiento 3, Parque Tecnológico de Ciencias de la Salud, E-18100 Armilla, Granada, Spain. [email protected]
PMID: 22694270 DOI: 10.1021/np3000987
Abstract

Three new cyclic tetrapeptides (1-3) have been isolated from the crude fermentation extract of Onychocola sclerotica. The planar structures of 1-3 were elucidated by detailed spectroscopic analyses using one- and two-dimensional NMR experiments and high-resolution mass spectrometry. The absolute configuration of the amino acid residues in each cyclotetrapeptide was established by Marfey's method. Compounds 1-3 displayed activity as cardiac Calcium Channel blockers (Cav1.2) but did not inhibit the hERG Potassium Channel and were not cytotoxic. These peptides are the first secondary metabolites ever reported from fungi of the order Arachnomycetales.

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