Chemical constituents of Hericium erinaceum associated with the inhibitory activity against cellular senescence in human umbilical vascular endothelial cells

  • J Enzyme Inhib Med Chem. 2015 Dec;30(6):934-40. doi: 10.3109/14756366.2014.995181.
Hyung Jun Noh  1 ,  Hyo Hyun Yang  2  3 ,  Geum Soog Kim  1 ,  Seung Eun Lee  1 ,  Dae Young Lee  1 ,  Je Hun Choi  1 ,  Seung Yu Kim  1 ,  Eun Suk Lee  1 ,  Seung Heon Ji  1 ,  Ki Sung Kang  4 ,  Hye-Jin Park  5 ,  Jae-Ryong Kim  2  3 ,  Ki Hyun Kim  6
Affiliations
  • 1. a Department of Herbal Crop Research , National Institute of Horticultural & Herbal Science , RDA , Eumseoung , Korea .
  • 2. b Department of Biochemistry and Molecular Biology, College of Medicine , Yeungnam University , Daegu , Korea .
  • 3. c Aging-associated Vascular Disease Research Center, Department of Biochemistry and Molecular Biology, College of Medicine , Yeungnam University , Daegu , Korea .
  • 4. d College of Korean Medicine , Gachon University , Seongnam , Korea .
  • 5. e Department of Food Science and Biotechnology, College of BioNano Technology , Gachon University , Seongnam , Korea , and.
  • 6. f Natural Product Research Laboratory, School of Pharmacy , Sungkyunkwan University , Suwon , Korea.
PMID: 25676326 DOI: 10.3109/14756366.2014.995181
Abstract

Hericium erinaceum is an edible and medicinal mushroom widely used in Korea, Japan, and China. On the search for biologically active compounds supporting the medicinal usage, the MeOH Extract of the fruiting bodies of H. erinaceum was investigated for its chemical constituents. Six compounds were isolated and identified as hericenone D (1), (22E,24R)-5α,8α-epidioxyergosta-6,22-dien-3β-ol (2), erinacerin B (3), hericenone E (4), hericenone F (5) and isohericerin (6) by comparing their spectroscopic data with previously reported values. The inhibitory effects on adriamycin-induced Cellular Senescence in human dermal fibroblasts (HDFs) and human umbilical vein endothelial cells (HUVECs) of the isolates (1-6) were studied. Among the isolated compounds, ergosterol peroxide (2) reduced senescence associated β-galactosidase (SA-β-gal) activity increased in HUVECs treated with adriamycin. According to experimental data obtained, the active compound may inspire the development of a new pharmacologically useful substance to be used in the treatment and prevention of age-related diseases.

Keywords
Ergosterol peroxide; Hericium erinaceum; human dermal fibroblasts; human umbilical vein endothelial cells; senescence associated β-galactosidase activity.
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