Anti-diabetic activities of cis- and trans-2,3,5,4'-tetrahydroxystilbene 2-O-β-glucopyranoside from Polygonum multiflorum

  • Mol Nutr Food Res. 2017 Aug;61(8). doi: 10.1002/mnfr.201600871.
Wenping Tang  1  2 ,  Shiming Li  1 ,  Yue Liu  3 ,  Jia-Ching Wu  4 ,  Min-Hsiung Pan  4 ,  Mou-Tuan Huang  3 ,  Chi-Tang Ho  2
Affiliations
  • 1. Hubei Collaborative Innovation Center for the Characteristic Resources Exploitation of Dabie Mountains, Hubei Key Laboratory of Economic Forest Germplasm Improvement and Resources Comprehensive Utilization; College of Life Science, Huanggang Normal University, Huanggang, China.
  • 2. Department of Food Science, Rutgers-The State University of New Jersey, New Brunswick, NJ, USA.
  • 3. Department of Chemical Biology, Rutgers-The State University of New Jersey, Piscataway, NJ, USA.
  • 4. Institute of Food Science and Technology, National Taiwan University, Taipei, Taiwan.
PMID: 28054445 DOI: 10.1002/mnfr.201600871
Abstract

Scope: Functional foods can be used alone or in combination with existing therapies in preventing and treating type 2 diabetes (T2D). Trans-2,3,5,4'-tetrahydroxystilbene 2-O-β-glucopyranoside (trans-THSG), a dominant bioactive compound from Polygonum multiflorum (PM)-a popular medicinal food in Asia, has attracted increasing research interests due to its strong antioxidant activity. The content of naturally occurring cis-THSG (cis-2,3,5,4'-tetrahydroxystilbene 2-O-β-glucopyranoside) was very low in PM root, but was prepared in this study by mimicking the traditional process of PM. The anti-diabetic effects of trans- and cis-THSG were evaluated in T2D to search for more efficacious food ingredient(s).

Methods and results: Trans-THSG was chromatographically purified from PM roots and cis-THSG was prepared with our innovative process via exposure of trans-THSG to UV-light. The anti-diabetic effects of both THSGs were tested with HFD-induced male CF-1 diabetic mice. Cis-THSG was found more effective than trans-THSG in hypoglycemic effect and in ameliorating glucose intolerance and Insulin resistance. In HepG2 cells, cis-THSG also demonstrated more potent activity than trans-THSG in suppressing transcription of phosphoenopyruvate carboxykinase (PEPCK).

Conclusion: Cis-THSG can be an enriched bioactive ingredient in PM roots from post-processing and is significantly more effective against hyperglycemia than trans-THSG. One of the effective pathways was through inhibition of PEPCK.

Keywords
2,3,5,4′-tetrahydroxystilbene glycoside; Hypoglycemic activity; PEPCK; Polygonum multiflorum; Type 2 diabetes.
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