Anti-influenza A Virus Activity of Dendrobine and Its Mechanism of Action

  • J Agric Food Chem. 2017 May 10;65(18):3665-3674. doi: 10.1021/acs.jafc.7b00276.
Richan Li  1 ,  Teng Liu  1 ,  Miaomiao Liu  1 ,  Feimin Chen  1 ,  Shuwen Liu  1 ,  Jie Yang  1
Affiliations
  • 1. Guangdong Provincial Key Laboratory of New Drug Screening, Guangzhou Key laboratory of Drug Research for Emerging Virus Prevention and Treatment, School of Pharmaceutical Sciences, Southern Medical University , Guangzhou 510515, China.
PMID: 28417634 DOI: 10.1021/acs.jafc.7b00276
Abstract

Dendrobine, a major component of Dendrobium nobile, increasingly draws attention for its wide applications in health care. Here we explore potential effects of dendrobine against influenza A virus and elucidate the underlying mechanism. Our results indicated that dendrobine possessed Antiviral activity against influenza A viruses, including A/FM-1/1/47 (H1N1), A/Puerto Rico/8/34 H274Y (H1N1), and A/Aichi/2/68 (H3N2) with IC50 values of 3.39 ± 0.32, 2.16 ± 0.91, 5.32 ± 1.68 μg/mL, respectively. Mechanism studies revealed that dendrobine inhibited early steps in the viral replication cycle. Notably, dendrobine could bind to the highly conserved region of viral nucleoprotein (NP), subsequently restraining nuclear export of viral NP and its oligomerization. In conclusion, dendrobine shows potential to be developed as a promising agent to treat Influenza Virus infection. More importantly, the results provide invaluable information for the full application of the Traditional Chinese Medicine named "Shi Hu".

Keywords
Dendrobium nobile; antiviral; dendrobine; influenza A virus; viral nucleoprotein.
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