Transitional metaplasia in intestinal epithelium of rats submitted to intestinal cystoplasty and treatment with L -lysine

  • Acta Cir Bras. 2017 Apr;32(4):297-306. doi: 10.1590/s0102-865020170040000297.
Alessandra Marques Dos Santos  1 ,  Joao Paulo Ferreira Coelho  2 ,  Camila de Carvalho Juanes  2 ,  Rafael Barbosa de Azevedo  2 ,  Clara Araujo Diniz  3 ,  Francisco Vagnaldo Fechine Jamacaru  4 ,  Conceição Aparecida Dornelas  5
Affiliations
  • 1. Fellow Master degree, Postgraduate Program in Pathology, Department of Pathology, Universidade Federal do Ceará (UFC), Brazil. Intellectual and scientific content of the study, histopathological examinations, technical procedures, manuscript preparation.
  • 2. Graduate student, Scientific Iniciation Program, UFC, Fortaleza-CE, Brazil. Technical procedures.
  • 3. Graduate student, Scientific Iniciation Program, UFC, Fortaleza-CE, Brazil. Acquisition of data.
  • 4. PhD, Researcher, Núcleo de Pesquisa e Desenvolvimento de Medicamentos (NPDM), School of Medicine, UFC, Fortaleza-CE, Brazil. Analysis and interpretation of data, statistical analysis.
  • 5. PhD, Associate Professor, Department of Pathology, UFC, Fortaleza-CE, Brazil. Conception and design of the study, technical procedures, critical revision, supervised all phases of the study.
PMID: 28538804 DOI: 10.1590/s0102-865020170040000297
Abstract

Purpose:: To evaluated the effects of L-lysine on the intestinal and urothelial epithelia in cystoplasty in rats.

Methods:: Twenty-eight 9-week-old rats were assigned to 4 groups: Group A (n=8) cystoplasty followed by administration of L-lysine (150 mg/kg body weight by gavage) for 30 weeks; Group B (n=8) cystoplasty + water for 30 weeks; Group C (n=6) L-lysine for 30 weeks; Group D (n=6) water for 30 weeks.

Results:: On histopathology with hematoxylin and eosin, mild to moderate hyperplasia transitional was observed in at the site of anastomosis in all Animals submitted to cystoplasty (Groups A and B), but "transitional metaplasia" of the intestinal glandular epithelium was more accentuated in Group A (p=0.045). No inflammatory cells, dysplasia or abnormalities were observed. Staining with Alcian blue revealed a substantial reduction of goblet cells and mucins in the colon segment (Groups A and B).

Conclusion:: The administration of L-lysine to rats accelerated the development of transitional metaplasia in the epithelium of the colon segment in cystoplasty.

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