2'-Deoxyadenosine 5'-diphosphoribose is an endogenous TRPM2 superagonist

  • Nat Chem Biol. 2017 Sep;13(9):1036-1044. doi: 10.1038/nchembio.2415.
Ralf Fliegert  1 ,  Andreas Bauche  1 ,  Adriana-Michelle Wolf Pérez  1 ,  Joanna M Watt  2  3 ,  Monika D Rozewitz  1 ,  Riekje Winzer  1 ,  Mareike Janus  1 ,  Feng Gu  1 ,  Annette Rosche  1 ,  Angelika Harneit  1 ,  Marianne Flato  1 ,  Christelle Moreau  2 ,  Tanja Kirchberger  1 ,  Valerie Wolters  1 ,  Barry V L Potter  2  3 ,  Andreas H Guse  1
Affiliations
  • 1. The Calcium Signalling Group, Department of Biochemistry and Molecular Cell Biology, University Medical Centre Hamburg-Eppendorf, Hamburg, Germany.
  • 2. Wolfson Laboratory of Medicinal Chemistry, Department of Pharmacy and Pharmacology, University of Bath, Bath, UK.
  • 3. Medicinal Chemistry &Drug Discovery, Department of Pharmacology, University of Oxford, Oxford, UK.
PMID: 28671679 DOI: 10.1038/nchembio.2415
Abstract

Transient receptor potential melastatin 2 (TRPM2) is a ligand-gated CA2+-permeable nonselective cation channel. Whereas physiological stimuli, such as chemotactic agents, evoke controlled CA2+ signals via TRPM2, pathophysiological stimuli such as Reactive Oxygen Species and genotoxic stress result in prolonged TRPM2-mediated CA2+ entry and, consequently, Apoptosis. To date, adenosine 5'-diphosphoribose (ADPR) has been assumed to be the main agonist for TRPM2. Here we show that 2'-deoxy-ADPR was a significantly better TRPM2 agonist, inducing 10.4-fold higher whole-cell currents at saturation. Mechanistically, this increased activity was caused by a decreased rate of inactivation and higher average open probability. Using high-performance liquid chromatography (HPLC) and mass spectrometry, we detected endogenous 2'-deoxy-ADPR in Jurkat T lymphocytes. Consistently, cytosolic nicotinamide mononucleotide adenylyltransferase 2 (NMNAT-2) and nicotinamide adenine dinucleotide (NAD)-glycohydrolase CD38 sequentially catalyzed the synthesis of 2'-deoxy-ADPR from nicotinamide mononucleotide (NMN) and 2'-deoxy-ATP in vitro. Thus, 2'-deoxy-ADPR is an endogenous TRPM2 superagonist that may act as a cell signaling molecule.

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