Alliin attenuates 1, 3-dichloro-2-propanol-induced lipogenesis in HepG2 cells through activation of the AMP-activated protein kinase-dependent pathway

  • Life Sci. 2018 Feb 15;195:19-24. doi: 10.1016/j.lfs.2017.12.040.
Jing Lu  1 ,  Bijun Cheng  1 ,  Zhuoqun Meng  1 ,  Baochen Fang  1 ,  Tianjiao Li  2 ,  Maocheng Sun  1 ,  Meitong Liu  1 ,  Shuang Guan  3
Affiliations
  • 1. Food Science and Engineering College, Jilin University, Changchun, Jilin Province 130062, PR China.
  • 2. Food Engineering College, Jilin Agricultural Science and Technology University, Jilin, Jilin Province 130062, PR China.
  • 3. Food Science and Engineering College, Jilin University, Changchun, Jilin Province 130062, PR China. Electronic address: [email protected].
PMID: 29305303 DOI: 10.1016/j.lfs.2017.12.040
Abstract

Accumulating evidence reveals the association of 1, 3-dichloro-2-propanol (1, 3-DCP) exposure and lipogenesis. Alliin, the most abundant sulphur compound in garlic, has been demonstrated to exhibit hypoglycemic, antioxidant and anti-inflammatory activities. Here, we showed that alliin attenuated lipogenesis induced by 1,3-DCP and that the reduction was due to activation of the AMPK pathway. HepG2 cells exposed to 1,3-DCP exhibited significant increase of triglyceride(TG) and total Cholesterol(TC), and alliin reduced the accumulation. Most importantly, alliin could up-regulate the phosphorylation of AMPK and down-regulate protein and gene expressions of SREBP-1; FAS; SREBP-2;HMGCR in 1,3-DCP-induced HepG2 cells. The results demonstrated that alliin was effective on attenuating 1,3-DCP-induced lipogenesis via activation of the AMPK-SREBPs signaling pathway in HepG2 cells.

Keywords
1,3-DCP; AMPK; HMGCR; Lipogenesis; SACS; SREBP.
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