Ala-geninthiocin, a new broad spectrum thiopeptide antibiotic, produced by a marine Streptomyces sp. ICN19

  • J Antibiot (Tokyo). 2019 Feb;72(2):99-105. doi: 10.1038/s41429-018-0115-2.
Appadurai Muthamil Iniyan  1  2 ,  Enge Sudarman  3  4 ,  Joachim Wink  2  4 ,  Rajaretinam Rajesh Kannan  5 ,  Samuel Gnana Prakash Vincent  6
Affiliations
  • 1. International Centre for Nanobiotechnology (ICN), Centre for Marine Science and Technology (CMST), Manonmaniam Sundaranar University, Rajakkamangalam-629502, Kanyakumari District, Tamil Nadu, India.
  • 2. Microbial Strain Collection, Helmholtz Centre for Infection Research GmbH (HZI), Inhoffenstrasse 7, 38124, Braunschweig, Germany.
  • 3. Department Microbial Drugs, Helmholtz Centre for Infection Research GmbH (HZI), Inhoffenstrasse 7, 38124, Braunschweig, Germany.
  • 4. German Centre for Infection Research Association (DZIF), Partner site Hannover-Braunschweig, Inhoffenstrasse 7, 38124, Braunschweig, Germany.
  • 5. Molecular and Nanomedicine Research Unit, Centre for Nanoscience and Nanotechnology (CNSNT), Sathyabama Institute of Science and Technology, Jeppiaar Nagar, Chennai, 600119, TN, India.
  • 6. International Centre for Nanobiotechnology (ICN), Centre for Marine Science and Technology (CMST), Manonmaniam Sundaranar University, Rajakkamangalam-629502, Kanyakumari District, Tamil Nadu, India. [email protected].
PMID: 30356080 DOI: 10.1038/s41429-018-0115-2
Abstract

Bioassay-guided screening of Antibacterial compounds from the cultured marine Streptomyces sp. ICN19 provided Ala-geninthiocin (1), along with its known analogs geninthiocin (2) and Val-geninthiocin (3) and the indolocarbazole staurosporine (4). The structure of 1 was determined on the basis of 1D and 2D NMR spectra and ESI-HRMS. The absolute configurations of the amino acid residues were determined by enantioselective GC-MS analysis. Compound 1 exhibited potent activity against Gram-positive bacteria including Staphylococcus aureus, Bacillus subtilis, Mycobacterium smegmatis, and Micrococcus luteus, as well as cytotoxicity against A549 human lung carcinoma cells with an IC50 value of 6 nM.

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